Pefachrome® VIIa, Chromogenic Substrate for Factor VIIa

Application:
Highly sensitive chromogenic peptide substrate for factor VIIa. Determination of factor VIIa activity for research, in-process and quality control of factor VIIa preparations. 

Formula: CH₃SO₂-D-CHA-But-Arg-pNA · AcOH 

Principle:
CH₃SO₂-D-CHA-But-Arg-pNA + E ==> CH₃SO₂-D-CHA-But-Arg-OH + pNA + E
E = Enzyme (factor VIIa) 

Solubility: Up to 4 mM in dist. H₂O

MW: 670.8 

Storage: May be used by the expiry date given on the label when stored unopened, protected from moisture, in the dark, 2-8°C. Avoid contamination of the reagents by micro-organisms. Shipment of product does not require cooling during the time of transportation. 

Note: The sensitivity of this substrate for factor VIIa is significantly increased in the presence of tissue factor (TF). 

Kinetic data:

	KM	Vmax
without TF	5.0 mM	6.72 nmol/min
with TF Ratio VIIa / TF:≈1/10	0.97 mM	69.7 nmol/min

Material required but not provided:
Tissue factor (TF), buffer, dist. water

Tissue factor: 0.31 mg/ml

Buffer: 0.1 M Tris, 100 mM NaCl, 5 mM CaCl₂, 0.1% BSA, pH 8.4

Assay:
Suggested protocol for the determination of factor VIIa activity:
0.020 ml factor VIIa (0.025 mg/ml)
0.020 ml TF
=>2 min at 37 °C

1.760 ml buffer

0.200 ml Pefachrome® FVIIa (4 mM in dist. water)
=> Determination of ∆OD/min at 405 nm

Category: Research use only

Type: Substrate

Product Availability: Germany, Israel and Egypt

Manufacturer: DSM Nutritional Products Ltd. Branch Pentapharm, Switzerland