1-chain tPA, human, recombinant (frozen)

Description
Tissue Plasminogen Activator (tPA) is a protease of the S1 family (trypsin family) and is found in a wide variety of mammalian tissues, especially endothelial cells. tPA is secreted as a single chain precursor, which is cleaved to a two-chain form by plasmin. Both forms are catalytically active and have similar enzymatic properties in the presence of fibrin.

Preparation
The 1-chain tPA is the secretion product of a Chinese Hamster Ovary cell line that had been transfected with the cDNA for human tPA obtained from a human melanoma cell line.

Molecular Weight
About 70 kDa (as determined on SDS-PAGE).

Purity
>95% 1-chain tPA (70 kD), <5% 2-chain tPA via SDS-PAGE analysis under reducing conditions.


Specific Activity
The specific activity is approximately 580,000 IU/mg.

Presentation
100 µg (ADG173) or 1 mg (ADG171) of sterile filtered single-chain recombinant tPA in a frozen solution of 34 mg/mL (3.4%) L-arginine, 10 mg/mL (1%) phosphoric acid.

Storage
Store frozen at -80°C. It is recommended to avoid freeze-thaw cycles.

Category: Research use only

Type: Protein

Product Availability: Worldwide

Manufacturer: ImmBioMed GmbH & Co KG, Germany

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