anti-human CD23, Clone LT-4F1
Description
CD23 (Fc epsilon RII), the low affinity IgE receptor, is a 45 kDa type II membrane glycoprotein expressed more or less on eosinophils, follicular dendritic cells, Langerhans cells, mature B cells (mainly upon activation), EBVtransformed lymphoblasts, monocytes, and subpopulation of platelets. A soluble form of 37 kDa and other its fragments were also described. CD23 mediates IgE-dependent cytotoxicity by eosinophils and macrophages, and downregulates IgE secretion in response to high levels of IgE, involving release of proinflammatory cytokines.
Properties
The monoclonal antibody ADG5015/L (clone LT-4F1) is a murine monoclonal antibody, subclass IgG1. The antibody has been purified form ascites by DEAE chromatography, Purity > 95% (by SDS-PAGE).
The antibody recognizes an epitope located in the stalk region of human low affinity IgE receptor (CD23) between the 37 and 25 kDa cleavage sites.
Presentation
Vial containing 100 µg /100 µl (ADG5015) or 300 µg/ 300 µl (ADG5015L) of purified antibody in PBS containing 0.09 % sodium azide (pH 7.2). The IgG concentration is 1 mg/ml. Spin the vial briefly before opening.
Storage and Stability
Store at 4 °C. For longterm storage aliquot and store at -20°. It is recommended to avoid freeze-thaw cycles. The reagent is stable until the expiry date stated on the vial label.
Applications
Flow Cytometry
Category: Research use only
Type: Antibody
Product Availability: Worldwide
Manufacturer: ImmBioMed GmbH & Co KG, Germany
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anti-human CD23, Clone LT-4F1
Cat.No. ADG5015
Artikelnr.: 938024
Einheit: 100 µg
Code: ADG5015
Hersteller: ImmBioMed GmbH & Co. KG
References
- Eur J Immunol. 1997 Sep;27(9):2212-8.
Expression and functions of the high-affinity IgE receptor on human platelets and megakaryocyte precursors.
Joseph M, Gounni AS, Kusnierz JP, Vorng H, Sarfati M, Kinet JP, Tonnel AB, Capron A, Capron M.
CD23 is known as the low-affinity IgE receptor (Fc epsilon RII). Binding of IgE to its receptors exerts cellular signaling. Platelets express both, the low-affinity IgE receptor (Fc epsilon RII/CD23), as well as the high-affinity receptor for IgE (Fc epsilon RI). By flow cytometric analysis the authors found that a low proportion of platelets co-expresses Fc epsilon RI and Fc epsilon RII/CD23. Functional studies revealed that anti-Fc epsilon RII/CD23 antibodies significantly inhibited Fc epsilon RI-mediated functions in platelets, indicating down-regulatory effects of receptor-ligand interaction of Fc epsilon RII/CD23.
- J Allergy Clin Immunol. 1997 Aug;100(2):235-41.
Endocytosis, storage, and release of IgE by human platelets: differences in patients with type I allergy and nonatopic subjects.
Klouche M, Klinger MH, Kühnel W, Wilhelm D.
The low-affinity IgE receptor (CD23) is expressed by platelets. Thus thrombocytes may contribute to long-lasting IgE sensitivity by serving as a storage pool for IgE. The authors provide evidence to conclude “that platelets of atopic individuals may contribute to allergic inflammation by serving as a storage pool for IgE and by their increased capacity to liberate further mediators of allergy in response to IgE stimulation.”
- Leukocyte Typing VI. Kishimoto T. et al. (Eds.), Garland Publishing Inc. (1997).
- Horejsi V. et al., Folia Biol. (Praha) 34, 23 (1988).
- Leucocyte Typing IV. Knapp W et al. (Eds.), Oxford University Press (1989)