anti-human CD63, Clone MEM-259

Description
CD63 (LAMP-3, lysosome-associated membrane protein-3), a glycoprotein of tetraspanin family, is present in late endosomes, lysosomes and secretory vesicles of various cell types. It is also present in the plasma membrane, usually following cell activation. Hence, it has become an widely used basophil activation marker. In mast cells, however, CD63 exposition does not need their activation. CD63 interacts with integrins and affects phagocytosis and cell migration, it is also involved in H/K-ATPase trafficking regulation of ROMK1 channels. CD63 also serves as a T-cell costimulation molecule. Expression of CD63 can be used for predicting the prognosis in earlier stages of carcinomas.

Properties
The monoclonal antibody ADG5068/L (clone MEM-259) is a murine monoclonal antibody, subclass IgG₁. The antibody has been purified from ascites using protein-A affinity chromatography, Purity > 95% (by SDS-PAGE).

The antibody reacts with CD63 (LAMP-3), a 40-60 kDa tetraspan glycoprotein expressed by granulo-cytes, platelets, T cells, monocytes/macrophages and endothelial cells. Cell surface exposition of CD63 is usu¬ally activation-dependent.

Presentation
Vial containing 100 µg /100 µl (ADG5068) or 300 µg/300 µl (ADG5068L) of purified antibody PBS containing 0.09 % sodium azide (pH 7.2) pH 7.2. The IgG concentration is 1 mg/ml. Spin the vial briefly before opening.

Storage and Stability
Store at 4 °C. For long-term storage aliquot and store at -20°. It is recommended to avoid freeze-thaw cycles. The reagent is stable until the expiry date stated on the vial label.

Applications
Flow Cytometry: detection of activated platelets, neutrophils and basophils
Immunoprecipitation
Western Blotting: non reducing condition

Category: Research use only

Type: Antibody

Product Availability: Worldwide

Manufacturer: ImmBioMed GmbH & Co KG, Germany

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