anti-human CD69, PE-conjugated, Clone FN50

Description
CD69 (C-type lectin domain family 2 C, CLEC2C, also known as AIM) is one of the earliest inducible cell surface molecules acquired during leukocyte activation. This glycoprotein serves as a lectin-type receptor in lymphocytes, NK cells and platelets; it is involved in lymphocyte proliferation. CD69 expression is counteracted on T cells in the AIDS stage of HIV infection, and may be also predictive for clinical response to chemoimmunotherapy.

Properties
The monoclonal antibody ADG5082/L (clone FN50) is a murine monoclonal antibody, subclass IgG₁ kappa. The antibody has been purified from cell culture supernatant using ion exchange chromatography, Purity > 95% (by SDS-PAGE).

The antibody recognizes the CD69 antigen, an early activation marker (within 2h) on T, B and NK-cells, possibly involved in signal transduction. It is also expressed by activated platelets. On flow cytometry the antibody stains > 90% of activated human peripheral blood lymphocytes. This antibody has been studied at the 4. International Workshop on Human Leucocyte Differentiation Antigens.

Conjugation
The purified antibody is conjugated with R-Phycoerythrin (R-PE) under optimum conditions. The reagent is adjusted for direct use. No reconstitution is necessary.

Presentation
Vial containing 500 µl (ADG5082) or 2 ml (ADG5082L) of purified antibody in PBS containing 1% BSA and 0.09% sodium azide (pH 7.2). The IgG concentration is 1 mg/ml. Spin the vial briefly before opening.

Storage and Stability
Store the antibody at 4°C. Avoid prolonged exposure to light. The reagent is stable until the expiry date stated on the vial label.

Applications
Flow cytometry.

Category: Research use only

Type: Antibody

Product Availability: Worldwide

Manufacturer: ImmBioMed GmbH & Co KG, Germany

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