murine MAb against human 12-Lipoxygenase (12-LOX/ALOX12), Clone 13.4

Description
12-Lipoxygenase (12-LOX) also known as Platelet-type 12-Lipoxygenase or arachindonate 12-lipoxygenase (ALOX12).

The enzyme acts on different polyunsaturated fatty acid substrates to generate bioactive lipid mediators including eicosanoids and lipoxins.

12-LOX protein plays an important role in inflammation and oxidation, while abnormal DNA methylation and genetic variants of 12-LOX are associated with various human diseases and pathological phenotypes, such as cardiovascular disease, diabetes, neurodegenerative diseases, respiratory system disease, cancer, infection, etc.

Properties
The antibody reacts with both native and recombinant 12-Lipoxygenase.

Preparation
The monoclonal antibody ADG0043 (clone 13.4) is a murine monoclonal antibody recognizing human 12-Lipoxygenase. Mice were immunized with purified recombinant full-length human 12-LOX, expressed in the baculovirus expression system. The antibody has been purified from cell culture supernatant using Protein A affinity chromatography.

Presentation
Vial containing 250 µg purified antibody in PBS pH 7.4. The concentration is given on the vial label. Spin the vial briefly before opening.

Storage and Stability
Store the antibody at 2°-8°C. For long-term storage the antibody should be aliquoted and stored at -20°C or colder. It is recommended to avoid freeze-thaw cycles.
 
Applications
ELISA
The antibody can be used as capture or detection antibody in ELISAs. An antibody concentration of 1-10 µg/ml is recommended.

Specificity
The antibody is specific for 12-LOX/ALOX12. No cross-reactivity was observed against other members of the Lipoxygenase family (5-LOX, 15-LOX-1, 15-LOX-2, 12R-LOX, and eLOX3) when tested in ELISA.

Category: Research use only

Type: Antibody

Product Availability: Worldwide

Manufacturer: ImmBioMed GmbH & Co KG, Germany

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