murine MAb against human 12-Lipoxygenase (12-LOX/ALOX12), Clone 28.52
Description
12-Lipoxygenase (12-LOX) also known as Platelet-type 12-Lipoxygenase or arachidonate 12-lipoxygenase (ALOX12).
The enzyme acts on different polyunsaturated fatty acid substrates to generate bioactive lipid mediators including eicosanoids and lipoxins.
12-LOX protein plays an important role in inflammation and oxidation, while abnormal DNA methylation and genetic variants of 12-LOX are associated with various human diseases and pathological phenotypes, such as cardiovascular disease, diabetes, neurodegenerative diseases, respiratory sysstem disease, cancer, infection, etc.
Properties
The antibody reacts with both native and recombinant 12-Lipoxygenase.
Preperation
The monoclonal antibody ADG0048 (clone no. 28.52) is a murine monoclonal antibody recognizing human 12-Lipoxygenase. Mice were immunized with purified recombinant full-length human 12-LOX, expressed in the baculovirus expression system. The antibody has been purified from cell culture supernatant using Protein A affinity chromategraphy.
Presentation
Vial containing 250 µg purified antibody in PBS pH 7.4. The concentration is given on the vial label. Spin the vial briefly before opening.
Storage and Stability
Store the antibody at 2°-8°C. For long-erm storage the antibody should be aliquoted and stored at -20°C or colder. It is recommended to avoid freeze-thaw cycles.
Applications
ELISA
The antibody can be used as capture or detection antibody in ELISAs. An antibody concentration of 1-10 µg/ml is recommended.
Western Blot
The antibody recognize native and recombinant human P-12-LOX following SDS-PAGE under reducing conditions. A primary antibody concentration of 1-10 µg/mL is recommended.
Flow Cytometry
The antibody can be used for flow cytometry on Formaldehyde-fixed and permeabilized human platelets. A primary antibody conc. of 1-10 µg/ml is recommended.
Category: Research use only
Type: Antibody
Product Availability: Worldwide
Manufacturer: ImmBioMed GmbH & Co KG, Germany
For more information please click .pdf icon below.
murine MAb against human 12-Lipoxygenase (12-LOX/ALOX12), Clone 28.52
Cat.No. ADG0048 (research box)
Artikelnr.: 938279
Einheit: 250 µg
Code: ADG0048
Hersteller: ImmBioMed GmbH & Co. KG - from the research box
References
- Human platelet 12-lipoxygenase, new findings about its activity, membrane binding and low-resolution structure. Aleem AM et al., E. J Mol Biol. 2008 Feb 8;376(1):193-209
- Platelet 12-lipoxygenase and stem cells in Barrett's esophagus. Jaśkiewicz K et al., Oncol Letters 2010 1: 789-791