murine MAb against outer membrane protein 18-kDa of Borrelia burgdorferi, Clone LA-82.1
Description
Lyme disease is the most common vector-borne disease in North America and Europe. The causative agent Borrelia burgdorferi is a bacterium that is maintained in an enzoonotic cycle between Ixodes ticks and a large range of mammals. The Western blot technique has been employed to analyze the humoral immune response in Lyme borreliosis and is used as a serodiagnostic confirmation test. The most important immunodominant proteins of Borrelia burgdorferi are the 94 kDa, 60 kDa, 41 kDa (flagellin), 34 kDa (Osp B), 31 kDa (Osp A), 30 kDa, 21 kDa (Osp C), 17/18 kDa, and 15 kDa proteins.
Properties
The monoclonal antibody ADG0113L (clone LA-82.1) is a murine monoclonal antibody, subclass IgG2a recognizing an outer membrane 18 kDa protein. Mice were immunized with cell lysates of Borrelia burgdorferi. The antibody has been purified from cell culture supernatant using Protein G affinity chromatography.
Presentation
Screw capped vial containing 1 mg of purified antibody in PBS pH 7.4. The IgG concentration is given on the vial label. Spin the vial briefly before opening.
Storage and Stability
Store the antibody at 2°-8°C. For long-term storage the antibody should be aliquoted and stored at –20°C or colder. It is recommended to avoid freeze-thaw cycles.
Applications
ELISA
The antibody can be used as capture antibody in ELISAs. An antibody concentration of 1-10 µg/ml is recommended.
Westernblot
The antibody is suitable for Western blot analysis, detecting native and recombinant lpLA7 following SDS-PAGE under reducing conditions. A primary antibody concentration of 1-10 µg/mL is recommended.
Immunocytochemistry
The antibody can be used for immune-cytochemistry on paraformaldehyde fixed.
Category: Research use only
Type: Antibody
Product Availability: Worldwide
Manufacturer: ImmBioMed GmbH & Co KG, Germany
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murine MAb against outer membrane protein 18-kDa of Borrelia burgdorferi, Clone LA-82.1
Cat.No. ADG0113L (research box)
Artikelnr.: 938299
Einheit: 1,0 mg
Code: ADG0113L
Hersteller: ImmBioMed GmbH & Co. KG - from the research box
References
- Western blot as a tool in the diagnosis of Lyme borreliosis. Zöller et al. Electrophoresis 1993; 14(9):937-944
- Serodiagnosis of Lyme borreliosis by western immunoblot: reactivity of various significant antibodies against Borrelia burgdorferi. Ma et al. J. Clin. Microbiol. 1992; 30(2):370-376
- Recombinant immunoblot in the serodiagnosis of Lyme borreliosis. Comparison with indirect immunofluorescence and enzyme-linked immunosorbent assay. Wilske et al. Med. Microbiol. Immunol. 1993; 182(5):255-270
- Genospecies and their influence on immunoblot results. Wilske et al. Wien. Klin. Wochenschr. 1998; 110(24):882-885
- Diagnostic value of proteins of three Borrelia species (Borrelia burgdorferi sensu lato) and implications for development and use of recombinant antigens for serodiagnosis of Lyme borreliosis in Europe. Hauser et al. Clin. Diagn. Lab. Immunol. 1998; 5(4):456-462