murine MAb against CihC of Borrelia recurrentis, Clone Brk2-47.1/BR2

Description
Borrelia recurrentis, the causal agent of louse-borne relapsing fever is transmitted to humans via infected body lice. Infection with B. recurrentis has been achieved only in humans and is accompanied by a systemic inflammatory disease, multiple relapses of fever and massive spirochetemia. A key virulence factor of B. recurrentis is their potential to undergo antigenic variation. In addition, B. recurrentis express a surface lipoprotein, termed CihC, and acquire C4b-binding protein (C4bp) and human C1 esterase inhibitor (C1-Inh), the major inhibitors of the classical and lectin pathway of complement activation to effectively evade innate and adaptive immunity.

Properties
The monoclonal antibody ADG0160L (Brk2-47.1/BR2) is a murine monoclonal antibody, subclass IgG₁ recognizing CihC of Borrelia recurrentis strain A1. Mice were immunized with rec. CihC. The antibody has been purified from cell culture supernatant using Protein G affinity chromatography.

Presentation
Screw capped vial containing 1 mg of purified antibody in PBS pH 7.4. The IgG concentration is given on the vial label. Spin the vial briefly before opening.

Storage and Stability
Store the antibody at 2°-8°C. For long-term storage the antibody should be aliquoted and stored at –20°C or colder. It is recommended to avoid freeze-thaw cycles.

Applications
ELISA
The antibody can be used as capture antibody in ELISAs. An antibody concentration of 1-10 µg/ml is recommended.
Westernblot
The antibody is suitable for Western blot analysis, detecting native and recombinant CihC following SDS-PAGE under reducing conditions. A primary antibody concentration of 1-10 µg/mL is recommended.
Immunocytochemistry
The antibody can be used for immunocytochemistry on paraformaldehyde fixed spirochetes.

Category: Research use only

Type: Antibody

Product Availability: Worldwide

Manufacturer: ImmBioMed GmbH & Co KG, Germany

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