murine MAb against HcpA of Borrelia recurrentis, Clone Br-4
Description
Borrelia (B.) recurrentis, the causal agent of louse-borne relapsing fever is transmitted to humans via infected body lice. Infection with B. recurrentis has been achieved only in humans and is accompanied by a systemic inflammatory disease, multiple relapses of fever and massive spirochetemia. A key virulence factor of B. recurrentis is their potential to undergo antigenic variation. In addition, B. recurrentis express a surface lipoprotein, termed HcpA, which by exploiting host proteins factor H, CFHR-1, and plasmin(ogen) confers resistance to both, complement attack and opsonization and simultaneously acquires an increased potential to invade host tissues.
Properties
The monoclonal antibody ADG0162L (Br-4) is a murine monoclonal antibody, subclass IgG2b recognizing HcpA of Borrelia recurrentis strain A1. Mice were immunized with rec. HcpA. The antibody has been purified from cell culture supernatant using Protein G affinity chromatography.
Presentation
Vial containing 1 mg purified antibody in PBS pH 7.4. The concentration is given on the vial label. Spin the vial briefly before opening.
Storage and Stability
Store the antibody at 2°-8°C. For long-term storage the antibody should be aliquoted and stored at –20°C or colder. It is recommended to avoid freeze-thaw cycles.
Applications
ELISA
The antibody can be used as capture antibody in ELISAs. An antibody concentration of 1-10 µg/ml is recommended.
Westernblot
The antibody is suitable for Western blot analysis, detecting native and recombinant HcpA following SDS-PAGE under reducing conditions. A primary antibody concentration of 1-10 µg/mL is recommended.
Immunocytochemistry
The antibody can be used for immunocytochemistry on paraformaldehyde fixed spirochetes.
Category: Research use only
Type: Antibody
Product Availability: Worldwide
Manufacturer: ImmBioMed GmbH & Co KG, Germany
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murine MAb against HcpA of Borrelia recurrentis, Clone Br-4
Cat.No. ADG0164L (research box)
Artikelnr.: 938327
Einheit: 1,0 mg
Code: ADG0162L
Hersteller: ImmBioMed GmbH & Co. KG - from the research box
References
- Borrelia recurrentis employs a novel multifunctional surface protein with anti-complement, anti-opsonic and invasive potential to escape innate immunity. Grosskinsky et al. PloS One. 2009; 4(3):e4858
- Human complement regulators C4b-binding protein and C1 esterase inhibitor interact with a novel outer surface protein of Borrelia recurrentis. Grosskinsky et al. PLoS Negl. Trop. Dis. 2011; 4(6):e698
- Immune evasion strategies of relapsing fever spirochetes. Rötterding and Kraiczy. Front. Immunol. 2020; 11:1560