murine MAb against BhCRASP-1 of Borrelia hermsii, Clone Bh-2.5
Description
Tick-borne relapsing fever in North America is primarily caused by the spirochete Borrelia hermsii. Spirochetes are transmitted to humans through the bite of infected soft ticks, in particular Ornithodoros hermsii. The pathogen employs multiple strategies, including the acquisition of complement regulators and antigenic variation, to escape innate and adaptive immune responses and to persist in the blood. The outer surface protein BhCRASP-1 binds the complement regulators factor H and FH-related protein 1. Furthermore, BhCRASP-1 binds plasminogen/plasmin and contributes to dissemination/invasion of B. hermsii.
Properties
The monoclonal antibody ADG0171L (Bh-2.5) is a murine monoclonal antibody, subclass IgG1 recognizing BhCRASP-1 of Borrelia hermsii strain HS1. Mice were immunized with rec. BhCRASP-1. The antibody has been purified from cell culture supernatant using Protein G affinity chromatography.
Presentation
Vial containing 1 mg purified antibody in PBS pH 7.4. The concentration is given on the vial label. Spin the vial briefly before opening.
Storage and Stability
Store the antibody at 2°-8°C. For long-term storage the antibody should be aliquoted and stored at –20°C or colder. It is recommended to avoid freeze-thaw cycles.
Applications
ELISA
The antibody can be used as capture antibody in ELISAs. An antibody concentration of 1-10 µg/ml is recommended.
Immunocytochemistry
The antibody can be used for immune-cytochemistry on paraformaldehyde fixed spirochetes.
Westernblot
The antibody is suitable for Western blot analysis, detecting native and recombinant BhCRASP-1 following SDS-PAGE under reducing conditions. A primary antibody concentration of 1-10 µg/mL is recommended.
Surface exposition of BhCRASP-1. A, B. hermsii after in-cubation with the BhCRASP-1-specific mAb (upper panel) and the flagellin-specific mAb LA21 (lower panel) followed by rabbit anti-mouse Cy3-conjugated IgG; for counterstaining, the DNA-binding 4,6-diamidino-2-phenylindole was used (middle), and a differential interference contrast image is also shown (left). B, Proteinase K treatment affects the surface expression of native BhCRASP-1. B. hermsii cells were incubated with the indicated concentration of proteinase K, lysedby sonication, immunoblotted, and screened with anti-BhCRASP-1 (Bh-2.5) and anti-FlaB (LA21) mAb.
Category: Research use only
Type: Antibody
Product Availability: Worldwide
Manufacturer: ImmBioMed GmbH & Co KG, Germany
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murine MAb against BhCRASP-1 of Borrelia hermsii, Clone Bh-2.5
Cat.No. ADG0171L (research box)
Artikelnr.: 938329
Einheit: 1,0 mg
Code: ADG0171L
Hersteller: ImmBioMed GmbH & Co. KG - from the research box
References
- Dual binding specificity of a Borrelia hermsii-associated complement regulator-acquiring surface protein for factor H and plasminogen discloses a putative virulence factor of relapsing fever spirochetes. Rossmann et al. J. Immunol. 2007; 178 (11):7292-7301
- BhCRASP-1 of the replasing fever spirochete Borrelia hermsii is a factor H- and plasminogen-binding protein. Rossmann et al. Int. J. Med. Microbiol. 2008; 298(S1):272-283