murine MAb against tick saliva protein of 13kDa (Salp13) of Ixodes ricinus, clone II/64.1

Description
Mammals that have been infested by ticks acquire immunity to subsequent bites by the vector, a phenomenon known as tick immunity. The components of tick saliva have many effects that may aid the vector during engorgement and could be important in evading host responses. Reported activities of tick saliva include anticoagulant and immunosuppressive functions, such as inhibition of the complement cascade, impairment of NK cell activity, reduction of homocytotrophic and circulating antibody titers, repression of cytokine production, and inhibition of T lymphocyte proliferation. Salp13 represents a major Ixodes ricinus salivary antigen that elicit antibodies in the host.

Properties
The monoclonal antibody ADG0194L (II/64.1) is a murine monoclonal antibody, subclass IgG₁ recognizing saliva protein Salp13 of Ixodes ricinus and I. scapularis. Mice were immunized with rec. Salp13. The antibody has been purified from cell culture supernatant using Protein G affinity chromatography.

Presentation
Screw capped vial containing 1 mg of purified antibody in PBS pH 7.4. The IgG concentration is given on the vial label. Spin the vial briefly before opening.

Storage and Stability
Store the antibody at 2°-8°C. For long-term storage the antibody should be aliquoted and stored at –20°C or colder. It is recommended to avoid freeze-thaw cycles.

Applications
ELISA
The antibody can be used as capture antibody in ELISAs. An antibody concentration of 1-10 µg/ml is recommended.
Westernblot
The antibody is suitable for Western blot analysis, detecting native and recombinant Salp13 following SDS-PAGE under reducing conditions. A primary antibody concentration of 1-10 µg/mL is recommended.
Immunocytochemistry
The antibody can be used for immune-cytochemistry.

Category: Research use only

Type: Antibody

Product Availability: Worldwide

Manufacturer: ImmBioMed GmbH & Co KG, Germany

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