murine MAb against human plasminogen, clone PG6
Description
Full length Plasminogen comprises seven domains, a C-terminal chymotrypsin-like serine protease domain, a N-terminal Pan Apple domain (PAp) and five Kringle domains (KR 1-5).
Plasminogen is synthesized in the liver and circulates in two forms: Glu-Plasminogen and Lys-Plasminogen. In its native form Plg contains a glutamic acid residue at the N-terminus and this molecule is termed Glu-Plasminogen.
Native Glu-Plasminogen (88 KDa) is readily converted to Lys-plasminogen (83 KDa) by Plasmin hydrolysis of the Lys76-Lys77 peptide bond.
Properties
The monoclonal antibodies were generated against purified human Plasminogen. The antibodies have been purified from cell culture supernatant using Protein G affinity chromatography.
Presentation
Screw capped vial containing 0.5 mg of purified antibody in PBS pH 7.4. The IgG concentration is given on the vial label. Spin the vial briefly before opening.
Storage and Stability
Store the antibody at 2°-8°C. For long-term storage the antibody should be aliquoted and stored at –20°C or colder. It is recommended to avoid freeze-thaw cycles.
Specificity
ADG3643
This antibody (clone HD-PG1, isotype IgG1) reacts with Glu- and Lys-plasminogen, with LBSI and LBSII, as determined per Elisa. (1) The antibody is suitable for Western blot (1) and Immunohistochemistry on frozen sections. (2)
ADG3646
This antibody (clone HD-PG6, isotype IgG1) reacts with Glu-, Lys-plasminogen and LBSI not with LBSII, as determined per Elisa. The antibody is suitable for Immunohistochemistry on frozen sections. (2, 3)
ADG3652
This antibody (clone HD-PG12, isotype IgG1) reacts with the non-kringle domains of Glu- and Lys plasminogen, as determined per Elisa. (1) The antibody is suitable for Immunohistochemistry on frozen sections. (2, 3)
ADG3653
This antibody (clone HD-PG13, isotype IgG1) reacts speifically with Glu-plasminogen, as determined per Elisa. (1) The antibody is suitable for Immunohisto-chemistry on frozen sections. (2, 3)
ADG3659
This antibody (clone HD-PG19, isotype IgG2a) reacts reacts with the non-kringle domains of Glu- and Lys plasminogen, as determined per Elisa. It cross-reacts with bovine Glu-plasmingen. The antibody is anti-catalytic, as determined by radial fibrinolysis assay (1). The antibody is suitable for Immunoprecipitation. (4)
Category: Research use only
Type: Antibody
Product Availability: Worldwide
Manufacturer: ImmBioMed GmbH & Co KG, Germany
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murine MAb against human plasminogen, clone PG6
Cat.No. ADG3646
Artikelnr.: 938352
Einheit: 250 µg
Code: ADG3646
Hersteller: ImmBioMed GmbH & Co. KG
References
- Monoclonal antibodies against plasminogen activators and plasmin(ogen). MD Kramer, U Vettel, M Schmitt, J Reinartz, G Brunner, and A Meissauer. Fibrinolysis 1992;6, Suppl 4:103-111.
- Enhanced association of plasminogen/plasmin with lesional epidermis of bullous pemphigoid. Gissler HM, Simon MM, Kramer MD. Br J Dermatol. 1992 Sep;127(3):272-277.
- Immunohistochemical characterization of the plasminogen activator system in psoriatic epidermis. Gissler HM, Frank R, Kramer MD. Br J Dermatol. 1993 Jun;128(6):612-618.
- Plasminogen activation in lesional skin of Pemphigus vulgaris type Neumann. Reinartz J, Naher H, Mai H, Kramer MD. Arch Dermatol Res. 1993;284(8):432-439