IMUBIND® Vitronectin ELISA

INTENDED USE
The IMUBIND^® Vitronectin ELISA is an enzyme-linked immunosorbent assay for the quantitative determination of total Vitronectin in human plasma or serum or in any fluid where Vitronectin might be present.

EXPLANATION OF THE TEST
Human Vitronectin is a major plasma glycoprotein that exhibits multiple activities and functions as a cell adhesion molecule and regulator of coagulation. It contains the amino acid structural motif Arg-Gly-Asp (RGD), which is involved in cell attachment. Human Vitronectin circulates as a single-chain moiety of 75 kDa and a two-chain moiety of 65 kDa and 10 kDa.
Vitronectin belongs to the group of structurally and functionally homologous adhesive proteins (fibrinogen, fibronectin, Von Willebrand factor) that interact with platelets and the vessel wall in the early stages of blood clotting. When coated on surfaces, very low concentrations of Vitronectin promote endothelial cell attachment and induce spreading and migration of cells in a time- and concentration-dependent fashion.
Reduced Vitronectin plasma levels (up to 50 %) have been found in several patients suffering from disseminated intravascular coagulation and degenerative liver disease (e.g. liver cirrhosis). Also, vitronectin deposition is associated with atherosclerotic lesions.

PRINCIPLE OF THE METHOD
Diluted samples are added to microwells coated with a monoclonal antibody against Vitronectin. During an incubation period, Vitronectin present in the sample will bind to the antibody coated to the wells. Following a washing step, a horseradish peroxidase (HRP) conjugated rabbit anti-Vitronectin polyclonal antibody is added to the microwells and binds to the Vitronectin protein captured on the plate during a short incubation period.  Following another washing step, the addition of a perborate-3,3’-5,5’-tetramethylbenzidine (TMB) substrate and its subsequent reaction with the HRP present generates a blue colored solution.  The reaction is stopped by adding citrate stop solution, which turns the solution color yellow. Measuring the solution absorbance at 450 nm and extrapolating the value with those of a standard curve determines the level of Vitonectin in the diluted plasma sample.

Category: Research use only

Type: ELISA

Product Availability: Worldwide

Manufacturer: ImmBioMed GmbH & Co KG, Germany

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