murine MAb against human PAI-1, clone HD-PAI-1 14.1

Description
The monoclonal antibody ADG3786 (clone HD-PAI-1 14.1) is a murine monoclonal antibody, subclass IgG₁ recognizing the human Plasminogen Activator Inhibitor Type 1 (PAI-1). Mice were immunized with purified recombinant PAI-1, expressed in a prokaryotic expression system. The antibody has been purified from cell culture supernatant using Protein A affinity chromatography. The antibody reacts with both native and recombinant PAI-1

Presentation
Screw capped vial containing 250 µg of purified antibody in PBS pH 7.4 + 0.01% ProClin300. The IgG concentration is 1 mg/ml. Spin the vial briefly before opening.

Storage and Stability
Store the antibody at 2°-8°C. For long-term storage the antibody should be aliquoted and stored at –20°C or colder. It is recommended to avoid freeze-thaw cycles.

Specificity
The antibody recognizes both active and inactive forms of PAl-1, and in addition it recognizes PAI-1 complexed with urokinase, tissue-type plasminogen activators, or vitronectin. ^{(1, 2)}
The antibody is specific for human PAI-1, no cross-reactivity was observed against other members of the plasminogen activator system (PAI-2, uPA, or tPA).

Applications
ELISA
The antibody can be used as capture or detection antibody in ELISAs. ^{(1, 2)}
Western Blot
The antibody is suitable for immuno-blotting. It recognizes native (a) and recombinant (b) human PAI-1.

Immunohistochemistry
The antibody can be used for immunohistological staining of formalin-fixed, paraffin-embedded tissue sections.

Category: Research use only

Type: Antibody

Product Availability: Worldwide

Manufacturer: ImmBioMed GmbH & Co KG, Germany

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