goat anti-human tPA IgG

Description
Tissue-type plasminogen activator (tPA) is a serine protease found on endothelial cells, the cells that line the blood vessels. As an enzyme, it catalyzes the conversion of plasminogen to plasmin, the major enzyme responsible for clot breakdown.
The antibody is derived from the sera of goats immunized with recombinant human tPA. The IgG fraction of immunserum was isolated by affinity chromatography on Protein G-Sepharose.

Properties
The antibody binds to both single-chain and two-chain tPA, it does not bind to uPA (urokinase-type plasminogen).

Presentation
Screw-capped clear glass vial containing 1.0 mg of purified antibody lyophilized from PBS, 200 mM mannitol, pH 7.5.

Reconstitution
Add 1 mL of filtered deionized or distilled water to the vial. Agitate gently for 5 minutes.

Storage
Store unopened vials at 2 - 8 °C until the expiration date shown on label.
Store reconstituted antibody in aliquots at -20 °C or colder. Stable for at least 6 months; avoid repeated freeze and thaw cycles.
 
Applications
ELISA
The antibody can be used as capture or detection antibody in ELISAs.
Immunohistochemistry
The antibody is reactive for human tPA in acetone fixed frozen and paraffin-embedded tissue sections.
Western Blot Analysis
The antibody is suitable for Western blot analysis of tPA. Generally, a single band at approximately 70 kDa is visualized under non-reducing conditions and 2 bands at approximately 40 kDa and 30 kDa (the heavy chain and light chain of tPA) are visualized under reducing conditions.

Category: Research use only

Type: Antibody

Product Availability: Worldwide

Manufacturer: ImmBioMed GmbH & Co KG, Germany

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