murine MAb against OspC Borrelia burgdorferi, Clone LA-38.2
Description
Infection with Borrelia burgdorferi, the etiologic agent of Lyme borreliosis, is associated with an early and dominant humoral response to the spirochete's 23-kDa outer surface protein C (OspC). OspC is the major protein expressed on the surface of B. burgdorferi during the first stages of infection and induces a strong IgM immune response early on [1]. OspC is a virulence factor upregulated just prior transmission to the mammalian host and is indispensable for establishing infection. Therefore, it is an essential antigen to include in serodiagnostic assays for early Lyme disease [2,3].
Distinct OspC genotypes are correlated with niche preference in natural reservoir species and invasiveness, pathogenesis and clinical manifes-tations in humans [4].
Preparation
The monoclonal antibody ADG89 (clone LA-38.2) is a murine monoclonal antibody, subclass IgG2b recognizing OspC. Mice were immunized with recombinant OspC antigen. The antibody has been purified from cell culture supernatant using Protein G affinity chromatography.
Presentation
Screw capped vial containing 250 µg of purified antibody in PBS pH 7.4 + 0.01% ProClin300. Spin the vial briefly before opening. The IgG concentration is indicated on the vial label.
Storage and Stability
Store the antibody at 2°-8°C. For long-term storage the antibody should be aliquoted and stored at –20°C or colder. It is recommended to avoid freeze-thaw cycles.
Applications
• ELISA
• Westernblot
Category: Research use only
Type: Antibody
Product Availability: Worldwide
Manufacturer: ImmBioMed GmbH & Co KG, Germany
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References
- Immunological and molecular polymorphisms of OspC, an immunodominant major outer surface protein of Borrelia burgdorferi. Wilske B, et al.,Infect Immun. 1993;61:2182–2191.
- Recombinant OspC from Borrelia burgdorferi sensu stricto, B. afzelii and B. garinii in the serodiagnosis of Lyme borreliosis. Panelius J, et al., J Med Microbiol. 2002;51:731–739.
- Significantly improved accuracy of diagnosis of early Lyme disease by peptide enzyme-linked immunosorbent assay based on the borreliacidal antibody epitope of Borrelia burgdorferi OspC. Jobe DA et al., Clin Vaccine Immunol. 2008;15:981–985.
- Comparative genome hybridization reveals substantial variation among clinical isolates of Borrelia burgdorferi sensu stricto with different pathogenic properties. Terekhova D, et al., J Bacteriol. 2006;188:6124–6134.