murine MAb against OspA Borrelia burgdorferi, Clone LA-31.1
Description
Borrelia burgdorferi, the etiologic agent of Lyme disease, makes different major outer surface lipoproteins at different stages of its mouse–tick infectious cycle (1). Different major outer surface lipoproteins are made at different stages of the cycle and represent points of interaction between the bacteria and their hosts and vectors. Outer surface protein A (OspA) is synthesized and coats the bacterial surface when spirochetes are acquired by larval ticks feeding on an infected small mammal, and it continues to be made as the B. burgdorferi-infected ticks molt to the nymphal stage (2,3). Antibodies directed against OspA have been shown to protect animals and humans from infection with B. burgdorferi (4,5). An OspA vaccine was approved by the US Food and Drug Administration in December 1998 (5).
Preparation
The monoclonal antibody ADG83 (clone LA-31.1) is a murine monoclonal antibody, subclass IgG1 recognizing OspA. Mice were immunized with recombinant OspA antigen. The antibody has been purified from cell culture supernatant using Protein G affinity chromatography.
Presentation
Screw capped vial containing 250 µg of purified antibody in PBS pH 7.4 + 0.01% ProClin300. Spin the vial briefly before opening. The IgG concentration is indicated on the vial label.
Storage and Stability
Store the antibody at 2°-8°C. For long-term storage the antibody should be aliquoted and stored at –20°C or colder. It is recommended to avoid freeze-thaw cycles.
Applications
• ELISA
• Westernblot
Category: Research use only
Type: Antibody
Product Availability: Worldwide
Manufacturer: ImmBioMed GmbH & Co KG, Germany
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murine MAb against OspA Borrelia burgdorferi, Clone LA-31.1
Cat.No. ADG0083 (research box)
Artikelnr.: 938542
Einheit: 250 µg
Code: ADG0083
Hersteller: ImmBioMed GmbH & Co. KG - from the research box
References
- Stage-specific global alterations in the transcriptomes of Lyme disease spirochetes during tick feeding and following mammalian host adaptation. Iyer R et al., Mol Microbiol 2015, 95:
509–538.
- Expression of outer surface proteins A and C of Borrelia burgdorferi in Ixodes ricinus. Fingerle V et al., J Clin Microbiol 1995, 33:1867–1869.
Induction of an outer surface protein on Borrelia burgdorferi during tick feeding. Schwan TG et al., Proc Natl Acad Sci U S A 1995, 92:2909–2913.
- Recombinant outer surface protein A of Borrelia burgdorferi induces antibodies protective against spirochetal infection in mice. Simon MM et al., J Infect Dis 1991,164:123–132.
- Vaccination against Lyme disease with recombinant Borrelia burgdorferi outer-surface lipoprotein A with adjuvant. Steere A C et al., N Engl J Med 1998, 339:209–215.