murine MAb against OspA Borrelia burgdorferi, Clone LA-2.2, purified
Description
The outer surface Protein A (OspA) of Borrelia burgdorferi has been a focus of scientific scrutiny since its identification. This 31-kDa lipoprotein is localized on the surface and in the periplasmic space of B. burgdorferi. OspA coats the spirochetes from the time the enter ticks until they are transmitted to a mammal. Vaccination with OspA elicits antibody that can target spirochetes in the tick midgut during feeding and inhibit transmission to mammals. OspA was the primary component of the human LYMErix™ vaccine.
Properties
The monoclonal antibody ADG81 (clone LA-2.2) is a murine monoclonal antibody, subclass IgG2b recognizing OspA. Mice were immunized with cell lysates of Borrelia burgdorferi. The antibody has been purified from cell culture supernatant using Protein G affinity chromatography.
Presentation
Vial containing purified antibody (ADG81mAb) in PBS pH 7.4 or ascites (ADG81asc). The concentration/ amount is given on the vial label. Spin the vial briefly before opening.
Storage and Stability
Store the antibody at –20°C or colder. It is recommended to avoid freeze-thaw cycles.
Applications
ELISA
The antibody can be used as capture antibody in ELISAs. An antibody concentration of 1-10 µg/ml is recommended.
Westernblot
The antibody is suitable for Western blot analysis, detecting native and recombinant OspA following SDS-PAGE under reducing conditions. A primary antibody concentration of 1-10 µg/mL is recommended.
Immunocytochemistry
The antibody can be used for immunocytochemistry on paraformaldehyde fixed spirochetes and for cryo-electron tomography.
Confocal immunofluorescence microscopy
reveals an even distribution of OspA (red)
on GFP-expressing Borrelia burgdorferi
Category: Research use only
Type: Antibody
Product Availability: Worldwide
Manufacturer: ImmBioMed GmbH & Co KG, Germany
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References
- Cloning and sequencing of the gene endcoding the outer surface prtotein A (OspA) of a European Borrelia burgdorferi isolate. Wallich et al. Nucleic Acids Res. 1989; 17(21):8864
- Monoclonal antibodies specific for the Outer surface protein A (OspA) of Borrelia burgdorferi prevent Lyme borreliosis in severe combined immunodeficiency (scid) mice. Schaible et al. Proc. Natl. Acad. Sci. USA 1990; 87(10):3768-3772
- Vaccination against Lyme disease with recombinant Borrelia burgdorferi outer-surface lipoprotein A with adjuvant. Lyme Disease Vaccine Study Group. Steere et al. N. Engl. J. Med. 1998; 339(4):209-215
- Structural identification of a key protective B-cell epitope in Lyme disease antigen OspA. Ding et al. J. Mol. Biol. 2000; 302(5):1153-1164
- Nanoscopic localization of surface-exposed antigens of Borrelia burgdorferi. Lemgruber et al. Microsc. Microanal. 2015; 21(03):680-688
- Comparative cryo‐electron tomography of pathogenic Lyme disease spirochetes. Kudryashev et al. Mol. Microbiol. 2009; 71(6):1415-1434