murine MAb against OspB Borrelia burgdorferi, Clone LA-25.1
Description
Lyme disease is the most common vector-borne disease in North America and Europe. The causative agent Borrelia burgdorferi is a bacterium that is maintained in an enzoonotic cycle between Ixodes ticks and a large range of mammals. The OspB gene is localized on the same 49-kb linear plasmid as OspA, and both genes are transcribed from a common promoter. B. burgdorferi upregulates OspA and OspB during entry into ticks. OspA and OspB contribute to the colonization of bacterium within the vector gut and facilitate survival of B. burgdorferi in ticks.
Properties
The monoclonal antibody ADG0084L (clone LA-25.1) is a murine monoclonal antibody, subclass IgG2b recognizing OspB. Mice were immunized with cell lysates of Borrelia burgdorferi. The antibody has been purified from cell culture supernatant using Protein G affinity chromatography.
Presentation
Vial containing 1 mg purified antibody in PBS pH 7.4. The concentration is given on the vial label. Spin the vial briefly before opening.
Storage and Stability
Store the antibody at 2°-8°C. For long-term storage the antibody should be aliquoted and stored at –20°C or colder. It is recommended to avoid freeze-thaw cycles.
Applications
ELISA
The antibody can be used as capture antibody in ELISAs. An antibody concentration of 1-10 µg/ml is recommended.
Westernblot
The antibody is suitable for Western blot analysis, detecting native and recombinant OspB following SDS-PAGE under reducing conditions. A primary antibody concentration of 1-10 µg/mL is recommended.
Immunocytochemistry
The antibody can be used for immunocytochemistry on paraformaldehyde fixed spirochetes and for cryo-electron tomography.
Wide-field immunofluorescence microscopy reveals a punctate distribution of OspB (red) on GFP-expressing Borrelia burgdorferi
Category: Research use only
Type: Antibody
Product Availability: Worldwide
Manufacturer: ImmBioMed GmbH & Co KG, Germany
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murine MAb against OspB Borrelia burgdorferi, Clone LA-25.1
Cat.No. ADG0084L (research box)
Artikelnr.: 938550
Einheit: 1,0 mg
Code: ADG0084L
Hersteller: ImmBioMed GmbH & Co. KG - from the research box
References
- Characterization of Borrelia burgdorferi associated antigens by monoclonal antibodies. Kramer et al. Immunobiol. 1990; 181:357-366
- Selection of an escape variant of Borrelia burgdorferi by use of bactericidal monoclonal antibodies to OspB. Coleman et al. Infect. Immun. 1992; 60(8):3098-3104
- Nanoscopic localization of surface-exposed antigens of Borrelia burgdorferi. Lemgruber et al. Microsc. Microanal. 2015; 21(3):680-688
- Complement-mediated in vitro bactericidal activity of monoclonal antibodies reactive with outer-surface-protein OspB of Borrelia burgdorferi. Cevenini et al. FEMS Microbiol. Lett. 1992; 69(2):147-152
- Molecular analysis of linear plasmid-encoded major surface proteins, OspA and OspB, of the Lyme disease spirochete Borrelia burgdorferi. Bergström et al. 1989; 3(4):479-486
- Selective association of outer surface lipoproteins with the lipid rafts of Borrelia burgdorferi. Toledo et al. 2014; 5(2):e00899-14