rabbit anti-human TFPI IgG

Description
Tissue factor pathway inhibitor (TFPI) is a plasma proteinase inhibitor synthesized by vascular endothelial cells.
Human TFPl is a modular protein comprised of three Kunitz type inhibitor domains flanked by peptide segments that are less structured.
The K1 domain inhibits factor Vlla complexed to tissue factor (TF) while the K2 domain inhibits Factor Xa. No direct protease Inhibiting functions have been demonstrated for the K3 domain. In plasma, TFPI is found both in a free-form (active) and in a lipoprotein-associated form (non-active).

Rabbits were immunized with purified human cell-derived, recombinant human TFPl. Total IgG was purified by protein A affinity chromato-graphy.

Properties
This antibody reacts with native and recombinant forms of TFPI.

Applications
A. Direct ELISA
This antibody can be used at 1 - 2 µg/mL with the appropriate secondary reagents to detect human TFPI.
B. Western Blot Analysis
This antibody can be used at 1 - 2 µg/mL with the appropriate secondary reagents to detect human TFPl in Western blot. ⁽⁵⁾
C. Flow Cytometry
The antibody can be used to detect surface-associated TFPI. ⁽⁵⁾
D. Immunofluorescence
The antibody can be used to stain formalin fixed cells. ⁽⁵⁾

Presentation
Screw capped vial containing 250 µg of purified antibody in PBS pH 7.4. The IgG concentration is 1 mg/ml.

Storage and Stability
Store the antibody at 2°-8°C. The antibody is preservative-free. Sodium azide or ProClin is recommended to avoid contamination (final concentration 0.01%-0.1%).
For long-term storage the antibody should be aliquoted and stored at –20°C or colder. It is recommended to avoid freeze-thaw cycles.

Category: Research use only

Type: Antibody

Product Availability: Worldwide

Manufacturer: ImmBioMed GmbH & Co KG, Germany

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