Factor XIII activity assay
INTENDED USE
Determination of Factor XIII activity in plasma samples.
ASSAY PRINCIPLE
Factor XIII is converted by Thrombin into Factor XIIIa. At the same time, Thrombin converts Fibrinogen into Fibrin. The clotting is prevented by an aggregation inhibiting peptide. Factor XIIIa cleaves a dark quenching molecule from the side chain of a modified peptide incorporating glycine methyl ester. Subsequently, the fluorescence of an N-terminal coupled dye increases and can be monitored on-line (excitation wavelength 313 nm; emission wavelength 418 nm). Basically, the isopeptidase activity of Factor XIIIa was described by Parameswaran et al. (1), the modified peptide used is intellectual property of Zedira (2,3).
TEST SAMPLE
Trisodium Citrate anticoagulated plasma
Category: Research use only
Type: ELISA
Product Availability: Worldwide
Manufacturer: ImmBioMed GmbH & Co KG, Germany
For more information please click .pdf icon below.
Factor XIII activity assay
Cat.No. ADG866
Artikelnr.: 938750
Einheit: 1 Kit
Code: ADG866
Hersteller: ImmBioMed GmbH & Co. KG
References
- Parameswaran KN, Cheng XF, Chen EC, Velasco PT, Wilson JH, Lorand L.; Hydrolysis of gamma:epsilon isopeptides by cytosolic transglutaminases and by coagulation factor XIIIa, J Biol Chem. 1997, 10311-7.
- Oertel K and Pasternack R; Fluorescence-based kinetic determination of the activity of Transglutaminases (EP04019090.2; US60/603,374).
- Oertel K, Hunfeld A, Specker E, Reiff C, Seitz R, Pasternack R, Dodt J; A highly sensitive fluorometric assay for determination of human coagulation factor XIII in plasma, Analytical Biochemistry 2007, 152-158.