IMUBIND TAT ELISA
INTENDED USE
The IMUBIND® TAT ELISA is an enzyme-linked immunosorbent assay for the quantitative determination of thrombin-antithrombin III (TAT) complexes.
EXPLANATION OF THE TEST
The conversion of prothrombin to thrombin is a key event in thrombus (clot) formation. Thrombin is a serine protease that acts on a wide variety of substrates in the coagulation pathway. Antithrombin III, an alpha 2 glycoprotein with a molecular weight of 58,000 kDa, is the major inhibitor of thrombin. Inactivation of thrombin by antithrombin III occurs by the formation of a covalent bond resulting in an inactive complex. This thrombin-antithrombin III complex (TAT) can be quantified in peripheral blood as an indicator of thrombin activation. Elevated plasma levels of TAT have been associated with a wide variety of active thrombotic events including disseminated intravascular coagulation (DIC), sepsis, multiple trauma, pregnancy complications (pre-eclampsia), deep vein thrombosis (DVT), and malignancies. TAT levels have also been found to be elevated in many patients with disturbances in global coagulation when routine coagulation parameters are in the sub-normal or normal range.
PRINCIPLE OF THE METHOD
Diluted plasma samples are added to microwells coated with a monoclonal antibody against thrombin. During an incubation period, TAT complexes present in the sample will bind to the antibody coated to the wells. Following a washing step, a biotinylated monoclonal anti-antithrombin III antibody is added to the microwells and binds to the TAT complexes captured on the plate during a short incubation period. A streptavidin-horseradish peroxidase conjugate (SA-HRP) is added to the microwells to complete the formation of the antibody-enzyme detection complex. Following another washing step, the addition of a perborate-3,3’-5,5’-tetramethylbenzidine (TMB) substrate and its subsequent reaction with the HRP present generates a blue colored solution. The reaction is stopped by adding citrate stop solution, which turns the solution color yellow. Measuring the solution absorbance at 450 nm and extrapolating the value with those of a standard curve determines the level of TAT in the diluted plasma sample.
Category: Research use only
Type: ELISA
Product Availability: Worldwide
Manufacturer: ImmBioMed GmbH & Co KG, Germany
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IMUBIND TAT ELISA
Cat.No. ADG833
Artikelnr.: 938831
Einheit: 96 Tests
Code: ADG833
Hersteller: ImmBioMed GmbH & Co. KG
BIBLIOGRAPHY
- Disseminated intravascular coagulation (DIC). Mammen EF. Clin Lab Sci. 2000 Fall;13(4):239-245.
- Useful lboratory tests for studying thrombogenesis in acute cardiac syndromes. Fareed J et al., Clin Chem. 1998 Aug;44(8 Pt 2):1845-1853.
- Contribution of the hemostasis laboratory in the diagnosis of deep venous thrombosis. Demarmels BF et al., Ther Umsch. 1996Apr;53(4):265-271.
- Derangements of coagulation and fibrinolysis in critically ill patients with sepsis and septic shock. Vervloet MG et al., Semin Thromb Hemost. 1998;24(1):33-44.