RVV-X (Russell's Viper Venom, factor X activating protein)

Description
Specific Factor X activator from Russell’s viper venom (RVV-X). RVV-X is a dimer of two peptide chains with a molecular weight of 60 kDa each. Activation of factor X by the activator strictly depends on the presence of calcium ions.

Applications
RVV-X is used in diagnostic procedures to quantitatively convert the zymogen factor X into factor Xa which can be determined by means of a clotting assay or photometrically, using a synthetic chromogenic substrate. RVV-X activator is used in testing of lupus anti-coagulants.

Assays
Chromogenic method
100 µl RVV-X (25 U/ml 25 mM CaCl₂)
10 µl human citrated plasma
=> incubate for 75 sec at 37°C to activate factor X
     into factor Xa
790 µl 50 mM Tris-HCl, pH 8.4
100 µl SPECTROZYME^® FXa substrate (4 mM)
=> Determination of ΔOD/min at 405 nm

Clotting method
100 µl RVV-X (5 mU/ml 20 mM Tris-HCl,      
                                          pH 7.2, 150 mM NaCl)
25 µl CaCl2 (25 mM)
25 µl rabbit brain cephaline (0.2 mg/ ml)
=> incubate for 1 min at 37 oC
25 µl human citrated plasma

Presentation
Clear screw-capped vial of 50 U purified Factor X activator from Russell's viper venom lyophilized in 20 mM Tris-HCl, 10 mM NaCl, 0.5% Prionex, pH 7.2. Reconstitute each vial with 1 ml filtered deionized water.
1 unit (U) is the amount of RVV-X which generates one international unit of enzyme (factor Xa) from zymogen (factor X) ⁽²⁾.

Storage and Stability
May be used by the expiry date given on the label when stored unopened, protected from moisture in the dark at 2°-8°C. Avoid contamination of the reagents by micro-organisms.

Stability after reconstitution:

The reconstituted product can be frozen immediately and stored at -20°C. Avoid refreezing.

Category: Research use only

Type: Snake venom protease activator

Product Availability: Worldwide

Manufacturer: ImmBioMed GmbH & Co KG, Germany

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