Pefachrome® FXIIa/TH 5253, Chromogenic Substrate for Thrombin

Application:
Highly sensitive chromogenic peptide substrate for factor XIIa. Determination of factor XIIa activity for research, in-process and quality control. 

Formula: H-D-CHA-Gly-Arg-pNA · 2AcOH

MW: 624.7 

Principle: H-D-CHA-Gly-Arg-pNA + FXIIa ==> H-D-CHA-Gly-Arg-OH + pNA + FXIIa 

KM: 0.8 mM

v_max:  3.14 µmol/min 

Solubility: Up to 4 mM in H₂O 

Storage:
May be used by the expiry date given on the label when stored unopened, protected from moisture, in the dark, 2-8°C. Avoid contamination of the reagents by micro-organisms. Shipment of product does not require cooling during the time of transportation. 

Material required but not provided: 
Buffer, NaCl, reference material, dist. H₂O, Pefabloc^® PK, Kalliplastin^®

Buffer:
50 mM Tris-imidazole buffer pH 7.9, 150 mM NaCl

Assay 1:
Suggested protocol for the determination of factor XIIa activity:
0.700 ml buffer 
0.100 ml factor XIIa (0.2 units/ml)
0.200 ml Pefachrome^®6017 4 mM
==> Determination of ∆OD/min at 405 nm

Assay 2:
Suggested protocol for the determination of factor XIIa activity (FXII activated by Kalliplastin^®) using Pefachrome^®6017 and a selective synthetic inhibitor of plasma kallikrein (Pefabloc^® PK):
0.100 ml citrated plasma (diluted 1:5 with NaCl)
0.200 ml Kalliplastin^® (20 µg/ml, Pentapharm Ltd.)
> incubate for 1 min at 37 °C
0.500 ml buffer
0.100 ml Pefabloc^® PK 0.2 mM
0.100 ml Pefachrome^®6017 2 mM
==> Determination of ∆OD/min at 405 nm

Category: Research use only

Type: Substrate

Product Availability: Germany, Israel and Egypt

Manufacturer: DSM Nutritional Products Ltd. Branch Pentapharm, Switzerland