Pefachrome® uPA 8294, Chromogenic Substrate for Urokinase (analog zu S-2444)

Application:
Highly sensitive chromogenic peptide substrate for urokinase (uPA). Determination of urokinase for research, in-process and quality control.

Formula: pyro-Glu-Gly-Arg-pNA · HCl

Principle: pyro-Glu Gly-Arg-pNA + uPA ==> pyro-Glu-Gly-Arg-OH + pNA + uPA

MW: 498.9

K_M: 6 µM

v_max: 1.3 · 10^-10 mol/min

Storage:
May be used by the expiry date given on the label when stored unopened, protected from moisture, in the dark, 2-8°C. Avoid contamination of the reagents by micro-organisms. Shipment of product does not require cooling during the time of transportation.

Material required but not provided:
Buffer, reference material

Buffer: 30 mM Tris-imidazole buffer pH 8.4, 300 mM NaCl

Assay:
Suggested protocol for the determination of urokinase activity:
1.740 ml buffer
0.060 ml Urokinase (approx. 2500 IU/ml, Calbiochem)
0.200 ml Pefachrome^®uPA 8294 (4 mM in water)
=> Determination of ∆OD/min at 405 nm, 37 °C

Category: Research use only

Type: Substrate

Product Availability: Germany, Israel and Egypt

Manufacturer: DSM Nutritional Products Ltd. Branch Pentapharm, Switzerland

For more information please click .pdf icon below.