Pefachrome® FXa/LAL 5288, Chromogenic Substrate for Factor Xa

Application:
Highly sensitive chromogenic peptide substrate for factor Xa. Determination of factor Xa activity for research, in-process and quality control of factor Xa preparations. 

Formula: CH₃OCO-D-CHA-Gly-Arg-pNA · AcOH 

Principle: CH₃OCO-D-CHA-Gly-Arg-pNA + FXa ==> CH₃OCO-D-CHA-Gly-Arg-OH + pNA+ FXa 

Solubility: Up to 4 mM in dist. H₂O

MW: 622.7 g/mol 

Kcat: 140 s^-1

KM: 0.106 mM 

Storage:
May be used by the expiry date given on the label when stored unopened, protected from moisture, in the dark, 2-8°C. Avoid contamination of the reagents by micro-organisms. Shipment of product does not require cooling during the time of transportation. 

Material required but not provided:
Buffer, 25 mM CaCl₂

Buffer: 50 mM Tris-HCl pH 8.4

RVV-X: 25 AU/ml in 25 mM CaCl₂

Micro assay:
Suggested protocol for the determination of factor X in plasma:
0.100 ml RVV-X
0.010 ml human citrated plasma
> incubate for 75 s at 37 °C - to activate factor X into factor Xa

0.790 ml buffer
0.100 ml Pefachrome^®FXa (4 mM in water)
=> Determination of ∆OD/min at 405 nm

Category: Research use only

Type: Substrate

Product Availability: Germany, Israel and Egypt

Manufacturer: DSM Nutritional Products Ltd. Branch Pentapharm, Switzerland