Pefachrome® PCa, Chromogenic Substrate for activated Protein C

Application:
Highly sensitive chromogenic peptide substrate for activated protein C. Determination of Protein Ca activity for research, in-process and quality control.

Formula: H-D-Lys(Cbo)-Pro-Arg-pNA · 2AcOH

Principle:
Protein C + Protac^® ==> Protein Ca
H-D-Lys(Cbo)-Pro-Arg-pNA + PCa ==> H-D-Lys(Cbo)-Pro-Arg-OH + pNA + PCa

Solubility: Up to 4 mM in H₂O

MW: 773.9

K_M: 0.303 mM

v_max: 25.0 µmol/ml protein C/min

Storage:
May be used by the expiry date given on the label when stored unopened, protected from moisture, in the dark, 2-8°C. Avoid contamination of the reagents by micro-organisms. Shipment of product does not require cooling during the time of transportation.

Material required but not provided:
Reference material, buffer, Protac^®

Buffer:
50 mM Tris-imidazole buffer pH 8.4, 150 mM NaCl

Assay:
Suggested protocol for the determination of Protac^®-activated protein C:
0.050 ml citrated plasma
0.100 ml Protac^®,0.5 units/ml
=> incubate for 5 min at 37°C

1.650 ml buffer 

0.200 ml Pefachrome^®PCa, 4 mM in H2O
==>Determination of ∆OD/min at 405 nm

Category: Research use only

Type: Substrate

Product Availability: Germany, Israel and Egypt

Manufacturer: DSM Nutritional Products Ltd. Branch Pentapharm, Switzerland