Pefachrome® VIIa, Chromogenic Substrate for Factor VIIa
Application:
Highly sensitive chromogenic peptide substrate for factor VIIa. Determination of factor VIIa activity for research, in-process and quality control of factor VIIa preparations.
Formula: CH3SO2-D-CHA-But-Arg-pNA · AcOH
Principle:
CH3SO2-D-CHA-But-Arg-pNA + E ==> CH3SO2-D-CHA-But-Arg-OH + pNA + E
E = Enzyme (factor VIIa)
Solubility: Up to 4 mM in dist. H2O
MW: 670.8
Storage: May be used by the expiry date given on the label when stored unopened, protected from moisture, in the dark, 2-8°C. Avoid contamination of the reagents by micro-organisms. Shipment of product does not require cooling during the time of transportation.
Note: The sensitivity of this substrate for factor VIIa is significantly increased in the presence of tissue factor (TF).
Kinetic data:
|
KM |
Vmax |
without TF |
5.0 mM |
6.72 nmol/min |
with TF Ratio VIIa / TF:≈1/10 |
0.97 mM |
69.7 nmol/min |
Material required but not provided:
Tissue factor (TF), buffer, dist. water
Tissue factor: 0.31 mg/ml
Buffer: 0.1 M Tris, 100 mM NaCl, 5 mM CaCl2, 0.1% BSA, pH 8.4
Assay:
Suggested protocol for the determination of factor VIIa activity:
0.020 ml factor VIIa (0.025 mg/ml)
0.020 ml TF
=>2 min at 37 °C
1.760 ml buffer
0.200 ml Pefachrome® FVIIa (4 mM in dist. water)
=> Determination of ∆OD/min at 405 nm
Category: Research use only
Type: Substrate
Product Availability: Germany, Israel and Egypt
Manufacturer: DSM Nutritional Products Ltd. Branch Pentapharm, Switzerland
For more information please click .pdf icon below.
Pefachrome VIIa (Pefa-5979)
Chromogenic Substrate for Factor VIIa
Article no.: 809301/25
Unit: 25 mg
Code: 093-01
Manufacturer: Pentapharm AG