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Pefachrome® VIIa, Chromogenic Substrate for Factor VIIa

Application:
Highly sensitive chromogenic peptide substrate for factor VIIa. Determination of factor VIIa activity for research, in-process and quality control of factor VIIa preparations. 

Formula: CH3SO2-D-CHA-But-Arg-pNA · AcOH 

Principle:
CH3SO2-D-CHA-But-Arg-pNA + E ==> CH3SO2-D-CHA-But-Arg-OH + pNA + E
E = Enzyme (factor VIIa) 

Solubility: Up to 4 mM in dist. H2O

MW: 670.8 

Storage: May be used by the expiry date given on the label when stored unopened, protected from moisture, in the dark, 2-8°C. Avoid contamination of the reagents by micro-organisms. Shipment of product does not require cooling during the time of transportation. 

Note: The sensitivity of this substrate for factor VIIa is significantly increased in the presence of tissue factor (TF). 

Kinetic data:
  KM Vmax
without TF 5.0 mM 6.72 nmol/min
with TF Ratio VIIa / TF:≈1/10       0.97 mM       69.7 nmol/min     

Material required but not provided:
Tissue factor (TF), buffer, dist. water

Tissue factor: 0.31 mg/ml

Buffer: 0.1 M Tris, 100 mM NaCl, 5 mM CaCl2, 0.1% BSA, pH 8.4

Assay:
Suggested protocol for the determination of factor VIIa activity:
0.020 ml factor VIIa (0.025 mg/ml)
0.020 ml TF
=>2 min at 37 °C
1.760 ml buffer
0.200 ml Pefachrome® FVIIa (4 mM in dist. water)
=> Determination of ∆OD/min at 405 nm

Category: Research use only

Type: Substrate

Product Availability: Germany, Israel and Egypt

Manufacturer: DSM Nutritional Products Ltd. Branch Pentapharm, Switzerland


For more information please click .pdf icon below.


Pefachrome VIIa (Pefa-5979)

Chromogenic Substrate for Factor VIIa
Article no.: 809301/25

Unit: 25 mg

Code: 093-01

Manufacturer: Pentapharm AG

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