RVV-X, Factor X Activator

Description:
Specific factor X activator from Russell’s viper venom. RVV-X is a dimer of two peptide chains with a molecular weight of 60’000 g/mol each. Activation of factor X by RVV-X strictly depends on the presence of calcium ions.

Application:
RVV-X is used in diagnostic procedures to quantitatively convert the zymogen factor X into factor Xa which can be determined by means of a clotting assay or photometrically, using a synthetic chromogenic substrate. RVV-X activator is used in testing of lupus anticoagulants.

Origin: Vipera russelli snake venom

MW: approx. 120’000

Storage:
May be used by the expiry date given on the label when stored unopened, protected from moisture, in the dark, 2°-8°C. Avoid contamination of the reagents by micro-organisms. Shipment of product does not require cooling during the time of transportation.

Assay:
Chromogenic method
100 µl RVV-X (25 U/ml 25 mM CaCl₂)
10 µl human citrated plasma
=> incubate for 75 s at 37 °C to
activate factor X into factor Xa
790 µl  50 mM Tris-HCl, pH 8.4
100 µl Pefachrome^®FXa (4 mM)
=> Determination of ∆OD/min at 405 nm

Clotting method
25 µl RVV-X (5 mU/ml 20 mM Tris­ HCl, pH 7.2, 150 mM NaCl)
25 µl CaCl₂ 25 mM
25 µl rabbit brain cephaline (0.2 mg/ ml) 
=> incubate for 1 min at 37 °C
25 µl  human citrated plasma

Unit definition:
1 unit (U) is the amount of RVV-X which generates one international unit of enzyme (factor Xa) from zymogen (factor X) [Stocker et al., 1986].

Stability after reconstitution:
+37°C  -  8 hours 
+15 to +25°C  -  2 days 
+2 to +8°C  -  1 week 
-80°C  -  1 month 

The reconstituted product can be frozen immediately and stored at -20°C. Avoid refreezing.

Category: Research use only

Type: Snake Venom Protease Activator

Product Availability: Germany, Israel and Egypt

Manufacturer: DSM Nutritional Products Ltd. Branch Pentapharm, Switzerland

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