Pefabloc® SC, Inhibitor for Proteinase
Description:
Pefabloc® SC is an irreversible proteinase inhibitor with broad specificity for serine proteinases.
Application:
Pefabloc® SC is suitable for biopharmaceutical downstream purification because of its superior solubility, stability, inhibitory activity and low toxicity.
Formula: C8H10FNO2S · HCl
MW: 239.7
Chem. Name:
4-(2-Aminoethyl)-benzenesulfonylfluoride hydrochloride (AEBSF)
Solubility: 200 mg/ml in H2O in water
Storage:
May be used by the expiry date given on the label when stored unopened, protected
from moisture, in the dark, 2-8°C. Avoid contamination of the reagents by micro
organisms.
Shipment of product does not require cooling during the time of transportation.
IC50 values:
The following table shows IC50 values for a selection of enzymes determined at pH 7.0, 25 °C after 15 minutes incubation time.
| Enzyme |
Enyzme conc. |
IC50 [mM] |
| Trypsin |
0.2 µg/ml |
0.081 |
| Chymotrypsin |
2.44 µg/ml |
0.044 |
| Thrombin |
0.079 µg/ml |
0.92 |
| Factor Xa |
0.12 U/ml |
24.0 |
| Plasmin |
4 CTA-U/ml |
1.99 |
| tPA |
5.0 µg/ml |
0.72 |
| uPA |
24.0 µg/ml |
0.072 |
| Glandular kallikrein |
3.5 µg/ml |
2.86 |
| Elastase |
2.44 µg/ml |
0.525 |
| Subtilisin A |
0.49 µg/ml |
1.801 |
| Factor XIIa |
0.013 U/ml |
0.256 |
Category: Research use only
Type: Inhibitor
Product Availability: Germany, Israel and Egypt
Manufacturer: DSM Nutritional Products Ltd. Branch Pentapharm, Switzerland
For more information please click .pdf icon below.
Pefabloc SC
Inhibitor for Proteinase
Article no.: 839901
Unit: 10 g
Code: 399-01
Manufacturer: Pentapharm AG
Pefabloc SC
Inhibitor for Proteinase
Article no.: 839901/g
Unit: 1 g
Code: 399-01
Manufacturer: Pentapharm AG
References:
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Plasmin, subtilisin-like endoproteases, tissue plasminogen activator, and urokinase plasminogen activator are involved in activation of latent TGF-β1 in human seminal plasma.
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Twodimensional zymography for analysis of proteolytic enzymes in human pure pancreatic juice.
Electrophoresis 1998; 19: 782-7.
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Migration of neutrophils across human pulmonary endothelial cells is not blocked by matrix metalloproteinase or serine protease inhibitors.
Am J Respir Cell Mol Biol 1999; 20: 1209-19.
- Mintz GR.
An irreversible serine protease inhibitor.
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A trypsin-like platelet protease propagates protease-activated receptor-1 cleavage and platelet activation.
Biochem J 1998; 336: 283-5.
- Salamone PR, Wodzinski RJ.
Production, purification and characterization of a 50-kDa extracellular metalloprotease from Serratia marcescens.
Appl Microbiol Biotechnol 1997; 48: 317-24.
- Winton HL, Wan H, Cannell MB, Thompson PJ, Garrod DR, Stewart GA, Robinson C.
Class specific inhibition of house dust mite proteinases which cleave cell adhesion, induce cell death and which increase the permeability of lung epithelium.
Brit J Pharmacol 1998; 124: 1048-59.