anti-human CD42b, PerCP-conjugated, Clone HIP1

Description
CD42b (GPIbα) composes together with GPIbβ, GPIX and GPV the GPIb-IX-V receptor complex critical in the process of platelet-rich thrombus formation by tethering the platelet to a thrombogenic surface. CD42b binds to von Willebrand factor (VWF) exposed at a site of vascular injury, as well as to thrombin, coagulation factors XI and XII, high molecular wight kininogen, TSP-1, integrin Mac-1 and P-selectin. The extracellular domain of CD42b by its interactions also contributes to metastasis.  

Properties
The monoclonal antibody ADG5038/L (clone HIP1) is a murine monoclonal antibody, subclass IgG₁. The antibody has been purified from by protein-G affinity chromatography, Purity > 95% (by SDS-PAGE).

The antibody recognizes a glycoprotein, heterodimer (GPIbα) which contains an α chain (CD42b, 145 kDa) and a β chain (CD42c, 22 kDa) linked by a disulfied bond forming a noncovalent complex with CD42a (GPIX) and d. This antigen is expressed on platelets and megakaryocytes. The GPIb/IX complex (CD42a-d) serves as a receptor for von Willebrand Factor (vWF) and thrombin, and mediates adhesion of platelets to subendothelium of damaged vascular walls. The absence of the CD42 complex leads to the Bernard-Soulier Syndrome (BSS). HIP1 McAv inhibits the ristocetin-dependent binding of vWF to platelets, ristocetin-induced platelets agglutination, and partially inhibits collagen-induced aggregation

Conjugation
The purified antibody is conjugated with Peridin Chlorophyll under optimum conditions. The reagent is adjusted for direct use. No reconstitution is necessary.

Presentation
Vial containing 500 µl (ADG5038) or 2 ml (ADG5038L) of purified antibody in PBS containing 1% BSA and 0.09% sodium azide (pH 7.2). The IgG concentration is 1 mg/ml. Spin the vial briefly before opening.

Storage and Stability
Store the antibody at 4°C. Avoid prolonged exposure to light. The reagent is stable until the expiry date stated on the vial label.

Applications
Flow Cytometry

Category: Research use only

Type: Antibody

Product Availability: Worldwide

Manufacturer: ImmBioMed GmbH & Co KG, Germany

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