anti-human CD45, PE/Dy647-conjugated, Clone MEM-28
Description
CD45 (LCA, leukocyte common antigen) is a receptor-type protein tyrosine phosphatase ubiquitously expressed in all nucleated hematopoietic cells, comprising approximately 10% of all surface proteins in lymphocytes. CD45 glycoprotein is crucial in lymphocyte development and antigen signaling, serving as an important regulator of Src-family kinases. CD45 protein exists as multiple isoforms as a result of alternative splicing; these isoforms differ in their extracellular domains, whereas they share identical transmembrane and cytoplasmic domains. These isoforms differ in their ability to translocate into the glycosphingolipid-enriched membrane domains and their expression depends on cell type and physiological state of the cell. Besides the role in immunoreceptor signaling, CD45 is important in promoting cell survival by modulating integrin-mediated signal transduction pathway and is also involved in DNA fragmentation during apoptosis.
Properties
The monoclonal antibody ADG5053/L (clone MEM-28) is a murine monoclonal antibody, subclass IgG1. The antibody has been purified from cell culture supernatant using protein-A affinity chromatography, Purity > 95% (by SDS-PAGE).
The antibody reacts with all alternative forms of human CD45 antigen (Leukocyte Common Antigen), a 180-220 kDa single chain type I transmembrane protein expressed at high level on all cells of hematopoietic origin, except erythrocytes and platelets.
Conjugation
The purified antibody is conjugated with PE-Dy647 under optimum conditions. The reagent is adjusted for direct use. No reconstitution is necessary.
Presentation
Vial containing 500 µl (ADG5053) or 2 ml (ADG5053L) of purified antibody in PBS containing 1% BSA and 0.09% sodium azide (pH 7.2). The IgG concentration is 1 mg/ml. Spin the vial briefly before opening.
Storage and Stability
Store the antibody at 4°C. Avoid prolonged exposure to light. The reagent is stable until the expiry date stated on the vial label.
Applications
Flow cytometry.
Category: Research use only
Type: Antibody
Product Availability: Worldwide
Manufacturer: ImmBioMed GmbH & Co KG, Germany
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anti-human CD45, PE/Dy647-conjugated, Clone MEM-28
Cat.No. ADG5053L
Article no.: 938126
Unit: 2 ml
Code: ADG5053L
Manufacturer: ImmBioMed GmbH & Co. KG
References
- Knapp W, et al. 1989. Leucocyte Typing IV. Oxford University Press. New York
- Kishihara K, et al. 1993. Cell 74:143.
Normal B lymphocyte development but impaired T cell maturation in CD45-exon6 protein tyrosine phosphatase-deficient mice.
- Esser M, et al. 2001. J. Virol. 75:6173. (WB)
Differential incorporation of CD45, CD80 (B7-1), CD86 (B7-2), and major histocompatibility complex class I and II molecules into human immunodeficiency virus type 1 virions and microvesicles: implications for viral pathogenesis and immune regulation.