Tissue factor, human, recombinant, liquid

Description
Tissue Factor (TF) is a 45 kDa transmembrane cell surface glycoprotein known for its role in initiating coagulation for the past 90 years.⁽¹⁾ TF is comprised of three domains: an extracellular domain (aa 1-219), followed by a hydrophilic spanning domain (aa 220-242) and a cytoplasmic tail (aa 243-263).⁽²⁾ It functions as a receptor and cofactor for the latent serine proteases factor VII and VIIa.^(3,4) Contact between TF and blood is sufficient to initiate the extrinsic pathway of coagulation.

Preparation
ADG45 is full length recombinant human tissue factor. The protein consists of amino acids 1-263, comprising the extracellular, transmembrane and cytoplasmic domains. It is produced by an E. coli expression system.

Purity
Estimated at greater than 98% per SDS gel electro-phoresis and silver staining. ADG45 appears as a single band at approximately 35 kDa.


Activity
The procoagulant activity was demonstrated in a chromogenic Tissue Factor activity assay (ACTICHROME^® TF assay, product no. 846).

Presentation
Screw capped vial containing 25 µg of purified human tissue factor in PBS pH 7.4. See vial label for lot-specific concentration.

Storage
Store frozen at -20°C or colder. It is recommended to avoid freeze-thaw cycles.

Category: Research use only

Type: Protein

Product Availability: Worldwide

Manufacturer: ImmBioMed GmbH & Co KG, Germany

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