IMUBIND® 12-Lipoxygenase ELISA
INTENDED USE
The IMUBIND® 12-Lipoxygenase (Platelet-type/arachidonate, 12-LOX, 12S-LOX, ALOX12) ELISA is an enzyme-linked immunosorbent assay for the quantitative determination of human 12-Lipoxygenase in human plasma or serum or in any fluid where 12-Lipoxygenase might be present.
EXPLANATION OF THE TEST
Human 12-Lipoxygenase is physiologically expressed in cells of the megakaryocytic lineage, in umbilical vein endothelium, and in epidermal cells. Its unique functions are the oxygenase and lipoxin synthase activities. 12-Lipoxygenase is localized to the cytoplasm.
Relevance
● 12-LOX is overexpressed in different types of cancers and plays an important role in cancer pathophysiology.
● Platelet-type 12-LOX is expressed in human melanoma cells of different origin, in their transplanted xenografts, and in fresh human skin tumors.
● Platelet-type 12-LOX is expressed in spontaneously metastasizing xenografts and in thick human skin tumors.
● Platelet 12-lipoxygenase (P-12-LOX) expression is elevated in prostate cancer and the level of expression is correlated with the grade of this cancer.
● Platelet-type 12-Lipoxygenase (12-LOX) has been shown to regulate growth, metastasis, and angiogenesis of prostate cancer.
PRINCIPLE OF THE METHOD
Diluted samples are added to microwells coated with a polyclonal antibody against 12-Lipoxygenase. During an incubation period, 12-Lipoxygenase present in the sample will bind to the antibody coated to the wells. Following a washing step, a horseradish peroxidase (HRP) conjugated anti-12-Lipoxygenase monoclonal antibody is added to the microwells and binds to the 12-Lipoxygenase protein captured on the plate during a short incubation period. Following another washing step, the addition of a perborate-3,3’-5,5’-tetramethylbenzidine (TMB) substrate and its subsequent reaction with the HRP present generates a blue colored solution. The reaction is stopped by adding citrate stop solution, which turns the solution color yellow. Measuring the solution absorbance at 450 nm and extrapolating the value with those of a standard curve determines the level of 12-Lipoxygenase in the diluted sample.
Category: Research use only
Type: ELISA
Product Availability: Worldwide
Manufacturer: ImmBioMed GmbH & Co KG, Germany
For more information please click .pdf icon below.
IMUBIND 12-Lipoxygenase ELISA
Cat.No. ADG872
Article no.: 938249
Unit: 96 Tests
Code: ADG872
Manufacturer: ImmBioMed GmbH & Co. KG
BIBLIOGRAPHY
- Human platelet 12-lipoxygenase, new findings about its activity, membrane binding and low-resolution structure. Aleem AM, Jankun J, Dignam JD, Walther M, Kühn H, Svergun DI, Skrzypczak-Jankun E. J Mol Biol. 2008 Feb 8;376(1):193-209
- Vascular endothelial growth factor production in human prostate cancer cells is stimulated by overexpression of platelet 12-lipoxygenase. McCabe NP, Selman SH, Jankun J. Prostate. 2006 May 15;66(7):779-787.
- Expression of cyclooxygenase-2 and 12-lipoxygenase in human breast cancer and their relationship with HER-2/neu and hormonal receptors: impact on prognosis and therapy. Mohammad AM, Abdel HA, Abdel W, Ahmed AM, Wael T, Eiman G. Indian J Cancer. 2006 Oct-Dec;43(4):163-168.
- Synthetic curcuminoids modulate the arachidonic acid metabolism of human platelet 12-lipoxygenase and reduce sprout formation of human endothelial cells. Jankun J, Aleem AM, Malgorzewicz S, Szkudlarek M, Zavodszky MI, Dewitt DL, Feig M, Selman SH, Skrzypczak-Jankun E. Mol Cancer Ther. 2006 May;5(5):1371-1382.
- Molecular identification, localization and function of platelet-type 12-lipoxygenase in human melanoma progression, under experimental and clinical conditions. Rásó E, Döme B, Somlai B, Zacharek A, Hagmann W, Honn KV, Tímár J. Melanoma Res. 2004 Aug;14(4):245-50.
- Platelet-type 12-lipoxygenase activates NF-kappaB in prostate cancer cells. Kandouz M, Nie D, Pidgeon GP, Krishnamoorthy S, Maddipati KR, Honn KV. Prostaglandins Other Lipid Mediat. 2003 Jul;71(3-4):189-204.
- Expression of 12-lipoxygenase as a biomarker for melanoma carcinogenesis. Winer I, Normolle DP, Shureiqi I, Sondak VK, Johnson T, Su L, Brenner DE. Melanoma Res. 2002 Oct;12(5):429-34.
- Expression, subcellular localization and putative function of platelet-type 12-lipoxygenase in human prostate cancer cell lines of different metastatic potential. Timár J, Rásó E, Döme B, Li L, Grignon D, Nie D, Honn KV, Hagmann W. Int J Cancer. 2000 Jul 1;87(1):37-43.