murine MAb against human 12-Lipoxygenase (12-LOX/ALOX12), Clone 25.20

Description
12-Lipoxygenase (12-LOX) also known as Platelet-type 12 Lipoxygenase or arachidonate 12-lipoxygenase (ALOX12)

The enzyme acts on different polyunsaturated fatty acid substrates to generate bioactive lipid mediators including eicosanoids and lipoxins.

12-LOX protein plays an important role in inflammation and oxidation, while abnormal DNA methylation and genetic variants of 12-LOX are associated with various humen diseases and pathological phenotypes, such as cardiovascular disease, diabetes, neurodegenerative diseases, respiratory system disease, cancer, infection, etc.

Properties
The antibody reacts with both native and recombinant 12-Lipoxygenase.

Preparation
The monoclonal antibody ADG0045 (clone no. 25.20) is a murine monoclonal antibody recognizing human 12-Lipoxygenase. Mice were immunized with purified recominant full-length human 12-LOX, expressed in the baculovirus expression system. The antibody has been purified from cell culture supernatant using Protein A affinity chromategraphy.

Presentation
Vial containing 250 µg purified antibody in PBS pH 7.4. The concentration is given on the vial label. Spin the vial briefly before opening.

Storage and Stability
Store the antibody at 2°-8°C. For long-term storage the antibody should be aliquoted and stored at –20°C or colder. It is recommended to avoid freeze-thaw cycles.

Specificity
The antibody is specific for 12-LOX/ALOX12. No cross-reactivity was observed against other members of the Lipoxygenase family (5-LOX, 15-LOX-1, 15-LOX-2, 12R-LOX, and eLOX3) when tested in ELISA and native PAGE.

Applications
ELISA
The antibody can be used as capture antibody in ELISAs. An antibody concentration of 1-10 µg/ml is recommended.
Western Blot
The antibody recognize native and recombinant human P-12-LOX following SDS-PAGE under reducing conditions. A primary antibody concentration of 1-10 µg/mL is recommended.

Immunohistochemistry
The antibody can be used for immunohistochemistry on formalin-fixed, paraffin-embedded tissue sections. A primary antibody concentration of 1-10 µg/ml is recommended. Optimal staining results were obtained using heat-induced antigen retrieval (30 min, 60°C) using a Streptavidin Peroxidase-visualisation system.

Category: Research use only

Type: Antibody

Product Availability: Worldwide

Manufacturer: ImmBioMed GmbH & Co KG, Germany

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