Factor Va, bovine, native

Description
Factor Va is a cofactor for the serine protease factor Xa, and in the presence of calcium ions they collectively assemble on a phospholipid surface to form the prothrombinase complex. The prothrombinase complex is responsible for the rapid conversion of prothrombin to thrombin. Factor Va is derived from the pro-cofactor, factor V, upon limited proteolysis by alpha-thrombin.
Factor Va is prepared by activating purified bovine factor V with thrombin and is subsequently purified by immunoaffinity chromatography.

Presentation
1 vial containing 100 μg of protein in a 50% glycerol/H2O/ 5 mM Calcium Chloride (vol/vol).

Storage and Stability
Store frozen at -20°C. Do not subject to freeze-thaw cycles! Spin briefly before opening

Properties
The thrombin cleavage of factor V liberates two heavily glycosylated activation peptides from the central portion of the molecule which have no cofactor function. Factor Va is comprised of an NH2-terminal derived heavy chain (94 kDa) and a COOH-terminal derived light chain (74 kDa) which remain associated in the presence of calcium ions.
Molecular weight: 168,000 Da
Purity: >95%
Extinction coefficient: 17.4
Concentration: see vial label
Specific activity*: see vial label

* Specific activity is determined by Factor V clotting assay (PT). One unit of activity is equivalent to the factor V activity in one milliter of normal plasma

Category: Research use only

Type: Protein

Product Availability: Worldwide

Manufacturer: ImmBioMed GmbH & Co KG, Germany

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