murine MAb against the 41-kDa flagellin of Borrelia burgdorferi, Clone LA-85.1
Description
Lyme disease is the most common vector-borne disease in North America and Europe. The causative agent Borrelia burgdorferi is a bacterium that is maintained in an enzoonotic cycle between Ixodes ticks and a large range of mammals. The 41 kDa flagellin of Borrelia burgdorferi is a major component of periplasmic flagellar filament core and a good candidate for serodiagnosis in early stage of Lyme disease.
Properties
The monoclonal antibody ADG0094L (clone LA-85.1) is a murine monoclonal antibody, subclass IgG2a recognizing flagellin. Mice were immunized with cell lysates of Borrelia burgdorferi. The antibody has been purified from cell culture supernatant using Protein G affinity chromatography.
Presentation
Vial containing 1 mg purified antibody in PBS pH 7.4. The concentration is given on the vial label. Spin the vial briefly before opening.
Storage and Stability
Store the antibody at 2°-8°C. For long-term storage the antibody should be aliquoted and stored at –20°C or colder. It is recommended to avoid freeze-thaw cycles.
Applications
ELISA
The antibody can be used as capture antibody in ELISAs. An antibody concentration of 1-10 µg/ml is recommended.
Westernblot
The antibody is suitable for Western blot analysis, detecting native and recombinant lpLA7 following SDS-PAGE under reducing conditions. A primary antibody concentration of 1-10 µg/mL is recommended.
Immunocytochemistry
The antibody can be used for immunocytochemistry on paraformaldehyde fixed.
Category: Research use only
Type: Antibody
Product Availability: Worldwide
Manufacturer: ImmBioMed GmbH & Co KG, Germany
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murine MAb against the 41-kDa flagellin of Borrelia burgdorferi, Clone LA-85.1
Cat.No. ADG0094L (research box)
Article no.: 938288
Unit: 1,0 mg
Code: ADG0094L
Manufacturer: ImmBioMed GmbH & Co. KG - from the research box
References
- Characterization of Borrelia burgdorferi associated antigens by monoclonal antibodies. Kramer et al. Immunobiol. 1990; 181:357-366
- The Borrelia burgdorferi flagellum-associated 41-kilodalton antigen (flagellin): molecular cloning, expression, and amplification of the gene. Wallich et al. Infect. Immun. 1990; 58(6) 1711-1719
- Cellular immune reactivity to recombinant OspA and flagellin from Borrelia burgdorferi in patients with Lyme borreliosis. Complexity of humoral and cellular immune responses. Krause et al. J. Clin. Invest. 1992; 90(3):1077-1084
- Antigenic variation and strain heterogeneity in Borrelia spp. Wilske et al. Res. Microbiol. 1992; 143(6):583-596
- Evaluation of genetic divergence among Borrelia burgdorferi isolates by use of OspA, fla, HSP60, and HSP70 gene probes. Wallich et al. Infect. Immun. 1992; 60(11):4856-4866