murine MAb against membrane lipoprotein IpLA7 of Borrelia burgdorferi, Clone LA-7
Description
Lyme disease is the most common vector-borne disease in North America and Europe. The causative agent Borrelia burgdorferi is a bacterium that is maintained in an enzoonotic cycle between Ixodes ticks and a large range of mammals. lpLA7, an immunogenic outer membrane lipoprotein of Borrelia burgdorferi, produced during infection, has been shown to play a redundant role in mammalian infection. lpLA7 facilitates pathogen survival in all tested phases of the vector-specific spirochete life cycle, including tick-to-host transmission.
Properties
The monoclonal antibody ADG0115L (clone LA-7) is a murine monoclonal antibody, subclass IgG2a recognizing lpLA7. Mice were immunized with cell lysates of Borrelia burgdorferi. The antibody has been purified from cell culture supernatant using Protein G affinity chromatography.
Presentation
Screw capped vial containing 1 mg of purified antibody in PBS pH 7.4. The IgG concentration is given on the vial label. Spin the vial briefly before opening.
Storage and Stability
Store the antibody at 2°-8°C. For long-term storage the antibody should be aliquoted and stored at –20°C or colder. It is recommended to avoid freeze-thaw cycles.
Applications
ELISA
The antibody can be used as capture antibody in ELISAs. An antibody concentration of 1-10 µg/ml is recommended.
Westernblot
The antibody is suitable for Western blot analysis, detecting native and recombinant lpLA7 following SDS-PAGE under reducing conditions. A primary antibody concentration of 1-10 µg/mL is recommended.
Immunocytochemistry
The antibody can be used for immunocytochemistry on paraformaldehyde fixed spirochetes.
2D gel of Borrelia burgdorferi lipoproteins, as identified by biosynthetic labeling with [3H]palmitate. pLA7=lpLA7
Category: Research use only
Type: Antibody
Product Availability: Worldwide
Manufacturer: ImmBioMed GmbH & Co KG, Germany
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murine MAb against membrane lipoprotein IpLA7 of Borrelia burgdorferi, Clone LA-7
Cat.No. ADG0115L (research box)
Article no.: 938305
Unit: 1,0 mg
Code: ADG0115L
Manufacturer: ImmBioMed GmbH & Co. KG - from the research box
References
- Characterization of Borrelia burgdorferi associated antigens by monoclonal antibodies. Kramer et al. Immunobiol. 1990; 181:357-366
- Molecular and immunological characterization of a novel polymorphic lipoprotein of Borrelia burgdorferi. Wallich et al. Infect. Immun. 1993; 61(10):4158-4166
- The lipoprotein La7 contributes to Borrelia burgdorferi persistence in ticks and their transmission to naïve hosts. Yang et al. Microbes Infect. 2013; 15:729-737
- Immunolocalization of a 22 kDa protein (lpLA7, P22) of Borrelia burgdorferi. Grewe et al. FEMS Microbiol. Lett. 1996; 138:215-219
- Recombinant low-molecular-mass proteins pG and LA7 from Borrelia burgdorferi reveal low diagnostic sensitivity in an enzyme-linked immunosorbent assay. Rauer et al. 2001; 39(5): 2039-2040