murine MAb against outer surface lipoprotein BBA36 of Borrelia burgdorferi, Clone A36-D4

Description
Lyme disease is the most common vector-borne disease in North America and Europe. The causative agent Borrelia burgdorferi is a bacterium that is maintained in an enzoonotic cycle between Ixodes ticks and a large range of mammals. Several Borrelia burgdorferi proteins are upregulated by temperature- and/or mammalian host-specific signals as the spirochete is transmitted from ticks to mammals. BBA36 outer surface protein was found to be up-regulated during transmission and infection process and is immunogenic in mammals.

Properties
The monoclonal antibody ADG0119L (clone A36-D4) is a murine monoclonal antibody, subclass IgG₁ recognizing BBA36. Mice were immunized with rec. BBA36 of Borrelia burgdorferi. The antibody has been purified from cell culture supernatant using Protein G affinity chromatography.

Presentation
Screw capped vial containing 1 mg of purified antibody in PBS pH 7.4. The IgG concentration is given on the vial label. Spin the vial briefly before opening.

Storage and Stability
Store the antibody at 2°-8°C. For long-term storage the antibody should be aliquoted and stored at –20°C or colder. It is recommended to avoid freeze-thaw cycles.

Applications
ELISA
The antibody can be used as capture antibody in ELISAs. An antibody concentration of 1-10 µg/ml is recommended.
Westernblot
The antibody is suitable for Western blot analysis, detecting native and recombinant BBA36 following SDS-PAGE under reducing conditions. A primary antibody concentration of 1-10 µg/mL is recommended.
Immunocytochemistry
The antibody can be used for immunocytochemistry on paraformaldehyde fixed spirochetes.

Category: Research use only

Type: Antibody

Product Availability: Worldwide

Manufacturer: ImmBioMed GmbH & Co KG, Germany

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