murine MAb against BGA70 (zqa70) of Borrelia garinii, Clone 70-11/10.17
Description
Lyme disease is the most common vector-borne disease in North America and Europe. The causative agents of diverse Borrelia spp. are maintained in an enzoonotic cycle between Ixodes ticks and a large range of mammals. Several Borrelia outer surface proteins are upregulated by temperature- and/or mammalian host-specific signals as the spirochete is transmitted from ticks to mammals. BGA70 outer surface protein was found to be immunogenic in mammals.
Properties
The monoclonal antibody ADG0150L (clone 70-11/10.17) is a murine monoclonal antibody, subclass IgG1 recognizing BGA70. Mice were immunized with rec. BGA70 of Borrelia garinii. The antibody has been purified from cell culture supernatant using Protein G affinity chromatography.
Presentation
Vial containing 1 mg purified antibody in PBS pH 7.4. The concentration is given on the vial label. Spin the vial briefly before opening.
Storage and Stability
Store the antibody at 2°-8°C. For long-term storage the antibody should be aliquoted and stored at –20°C or colder. It is recommended to avoid freeze-thaw cycles.
Applications
ELISA
The antibody can be used as capture antibody in ELISAs. An antibody concentration of 1-10 µg/ml is recommended.
Westernblot
The antibody is suitable for Western blot analysis, detecting native and recombinant BGA70 following SDS-PAGE under reducing conditions. A primary antibody concentration of 1-10 µg/mL is recommended.
Immunocytochemistry
The antibody can be used for immune-cytochemistry on paraformaldehyde fixed spirochetes.
Category: Research use only
Type: Antibody
Product Availability: Worldwide
Manufacturer: ImmBioMed GmbH & Co KG, Germany
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murine MAb against BGA70 (zqa70) of Borrelia garinii, Clone 70-11/10.17
Cat.No. ADG0150L (research box)
Article no.: 938318
Unit: 1,0 mg
Code: ADG0150L
Manufacturer: ImmBioMed GmbH & Co. KG - from the research box
References
- Identification and functional characterization of complement regulator-acquiring surface protein 1 of the Lyme disease spirochetes Borrelia afzelii and Borrelia garinii. Wallich et al. Infect. Immun. 2005; 73(4):2351-2359
- Borrelia burgdorferi surface-localized proteins expressed during persistant murine infection are conserved among diverse Borrelia spp. Hughes et al. Infect. Immun. 2008; 76(6):2498-2511
- BBA70 of Borrelia burgdorferi is a novel plasminogen-binding protein. Koenigs et al. J. Biol. Chem. 2013; 288(35):25229-25243
- Evaluation of selected Borrelia burgdorferi lp54 plasmid-encoded gene products expressed during mammalian infection as antigens to improve serodiagnostic testing for early Lyme disease. Weiner et al. Clin. Vaccine Immunol. 2015; 22(11):1176-1186
- Evaluation of in vivo expressed Borrelia burgdorferi antigens for improvement IgM serodiagnosis of early Lyme disease. Brandt et al. Diagn. Microbiol. Infect. Dis. 2019; 93(3):196-202