murine MAb against BGA71 of Borrelia garinii, Clone 71-11/14.1
Description
Lyme disease is the most common vector-borne disease in North America and Europe. The causative agents of the Borrelia burgdorferi sensu lato complex are maintained in an enzoonotic cycle between Ixodes ticks and a large range of mammals. Several Borrelia outer surface proteins are upregulated by temperature- and/or mammalian host-specific signals as the spirochete is transmitted from ticks to mammals. BGA71 outer surface lipoprotein (CRASP) was found to be able to bind complement regulator FHL-1 and is immunogenic in mammals.
Properties
The monoclonal antibody ADG0151L (clone 71-11/14.1) is a murine monoclonal antibody, subclass IgG1 recognizing BGA71. Mice were immunized with rec. BGA71 of Borrelia garinii. The antibody has been purified from cell culture supernatant using Protein G affinity chromatography.
Presentation
Vial containing 1 mg purified antibody in PBS pH 7.4. The concentration is given on the vial label. Spin the vial briefly before opening.
Storage and Stability
Store the antibody at 2°-8°C. For long-term storage the antibody should be aliquoted and stored at –20°C or colder. It is recommended to avoid freeze-thaw cycles.
Applications
ELISA
The antibody can be used as capture antibody in ELISAs. An antibody concentration of 1-10 µg/ml is recommended.
Immunocytochemistry
The antibody can be used for immune-cytochemistry on paraformaldehyde fixed spirochetes.
Westernblot
The antibody is suitable for Western blot analysis, detecting native and recombinant BGA71 following SDS-PAGE under reducing conditions. A primary antibody concentration of 1-10 µg/mL is recommended.
Category: Research use only
Type: Antibody
Product Availability: Worldwide
Manufacturer: ImmBioMed GmbH & Co KG, Germany
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murine MAb against BGA71 of Borrelia garinii, Clone 71-11/14.1
Cat.No. ADG0151L (research box)
Article no.: 938319
Unit: 1,0 mg
Code: ADG0151L
Manufacturer: ImmBioMed GmbH & Co. KG - from the research box
References
- Identification and functional characterization of complement regulator-acquiring surface protein 1
of the Lyme disease spirochetes Borrelia afzelii
and Borrelia garinii. Wallich et al. Infect. Immun. 2005; 73(4):2351-2359
- Identification and functional characterization of complement regulator-acquiring surface protein-1
of serum resistant Borrelia garinii OspA serotype 4. Van Burgel et al. BMC Microbiol. 2010; 10:43
- BGA66 and BGA71 facilitate complement resistance of Borrelia bavariensis by inhibiting assembly of the outer membrane attack complex. Hammerschmidt et al. Mol. Microbiol. 2016; 99(2): 407-424
- Hide and seek: How Lyme disease spirochetes overcome complement attack. Kraiczy. Front. Immunol. 2016; 7:385
- Host immune evasion by Lyme and relapsing fever Borreliae: Findings to lead future studies for Borrelia miyamotoi. Stone and Brissette. Front. Immunol. 2017; 8:12
- Crystal structure of the membrane attack complex assembly inhibitor BGA71 from the Lyme disease agent Borrelia bavariensis. Brangulis et al. Sci. Rep. 2018; 8(1):11286