murine MAb against HcpA of Borrelia recurrentis, Clone Br-4

Description
Borrelia (B.) recurrentis, the causal agent of louse-borne relapsing fever is transmitted to humans via infected body lice. Infection with B. recurrentis has been achieved only in humans and is accompanied by a systemic inflammatory disease, multiple relapses of fever and massive spirochetemia. A key virulence factor of B. recurrentis is their potential to undergo antigenic variation. In addition, B. recurrentis express a surface lipoprotein, termed HcpA, which by exploiting host proteins factor H, CFHR-1, and plasmin(ogen) confers resistance to both, complement attack and opsonization and simultaneously acquires an increased potential to invade host tissues.

Properties
The monoclonal antibody ADG0162L (Br-4) is a murine monoclonal antibody, subclass IgG_2b recognizing HcpA of Borrelia recurrentis strain A1. Mice were immunized with rec. HcpA. The antibody has been purified from cell culture supernatant using Protein G affinity chromatography.

Presentation
Vial containing 1 mg purified antibody in PBS pH 7.4. The concentration is given on the vial label. Spin the vial briefly before opening.

Storage and Stability
Store the antibody at 2°-8°C. For long-term storage the antibody should be aliquoted and stored at –20°C or colder. It is recommended to avoid freeze-thaw cycles.

Applications
ELISA
The antibody can be used as capture antibody in ELISAs. An antibody concentration of 1-10 µg/ml is recommended.
Westernblot
The antibody is suitable for Western blot analysis, detecting native and recombinant HcpA following SDS-PAGE under reducing conditions. A primary antibody concentration of 1-10 µg/mL is recommended.
Immunocytochemistry
The antibody can be used for immunocytochemistry on paraformaldehyde fixed spirochetes.

Category: Research use only

Type: Antibody

Product Availability: Worldwide

Manufacturer: ImmBioMed GmbH & Co KG, Germany

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