murine MAb against BaCRASP-1/cspA of Borrelia afzelii, Clone BaC3/1.12
Description
Lyme disease is the most common vector-borne disease in North America and Europe. The causative agent Borrelia burgdorferi is a bacterium that is maintained in an enzoonotic cycle between Ixodes ticks and a large range of mammals. Adaptation to the diverse environmental conditions, including sophisticated means of evading the vertebrate hosts' immune system, in particular complement occurs at the first line of defense following infection. Borrelia burgdorferi sensu lato spirochetes express up to five complement regulator-acquiring surface proteins (CRASPs) binding human complement regulators.
Properties
The monoclonal antibody ADG0170L (clone BaC3/1.12) is a murine monoclonal antibody, subclass IgG2b recognizing BaCRASP-1. Mice were immunized with cell lysates of Borrelia afzeliii. The antibody has been purified from cell culture supernatant using Protein G affinity chromatography.
Presentation
Vial containing 1 mg purified antibody in PBS pH 7.4. The concentration is given on the vial label. Spin the vial briefly before opening.
Storage and Stability
Store the antibody at 2°-8°C. For long-term storage the antibody should be aliquoted and stored at –20°C or colder. It is recommended to avoid freeze-thaw cycles.
Applications
ELISA
The antibody can be used as capture antibody in ELISAs. An antibody concentration of 1-10 µg/ml is recommended.
Westernblot
The antibody is suitable for Western blot analysis, detecting native and recombinant BaCRASP-1 following SDS-PAGE under reducing conditions. A primary antibody concentration of 1-10 µg/ml is recommended.
Immunocytochemistry
The antibody can be used for immune-cytochemistry on paraformaldehyde fixed spirochetes.
Category: Research use only
Type: Antibody
Product Availability: Worldwide
Manufacturer: ImmBioMed GmbH & Co KG, Germany
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murine MAb against BaCRASP-1/cspA of Borrelia afzelii, Clone BaC3/1.12
Cat.No. ADG0170L (research box)
Article no.: 938328
Unit: 1,0 mg
Code: ADG0170L
Manufacturer: ImmBioMed GmbH & Co. KG - from the research box
References
- Immune evasion of Borrelia burgdorferi by acquisition of human complement regulators FHL-1/reconectin and Factor H. Kraiczy et al. Eur. J. Immunol. 2001; 31(6):1674-1684
- Complement resistance of Borrelia burgdorferi correlates with the expression of BbCRASP-1, a novel linear plasmid-encoded surface protein that interacts with human factor H and FHL-1 and is unrelated to Erp proteins. Kraiczy et al. J. Biol. Chem. 2004; 279(4):2421-2429
- Identification and functional characterization of complement regulator-acquiring surface protein 1 of the Lyme disease spirochetes Borrelia afzelii and Borrelia garinii. Wallich et al. Infect. Immun. 2005; 73(4):2351-2359
- Borrelia burgdorferi regulates expression of complement regulator-acquiring surface protein 1 during mammal-tick infection cycle. Von Lackum et al. Infect. Immun. 2005; 73(11):7398-7405
- Borrelia burgdorferi complement regulator-acquiring surface protein 1 of the Lyme disease spirochetes is expressed in humans and induces antibody responses restricted to nondenatured structural determinants. Rossmann et al. Infect. Immun. 2006; 74(12):7024-7028
- Identification and characterization of the factor H and FHL-1 binding complement regulator-acquiring surface protein 1 of the Lyme disease spirochete Borrelia spielmanii. Herzberger et al. Int. J. Med. Microbiol. 2009; 299(2):141-154