murine MAb against BpcA of Borrelia parkeri, Clone Bp-3/32.2

Description
Tick-borne relapsing fever (TBRF) in North America is caused by the spirochete species Borrelia hermsii, Borrelia parkeri and Borrelia turicatae, which are transmitted to humans through the bite of the respective infected Ornithodoros tick vector. The pathogen employs multiple strategies, including the acquisition of complement regulators and antigenic variation, to escape innate and adaptive immune responses and to persist in the blood. The outer surface lipoprotein BpcA binds the complement regulators factor H and FH-related protein 1 and, simultaneously, the host plasminogen/plasmin and may aid in the spirochete’s dissemination and invasion.

Properties
The monoclonal antibody ADG0173L (Bp-3/32-2) is a murine monoclonal antibody recognizing BpcA of Borrelia parkeri strain RML. Mice were immunized with rec. BpcA. The antibody has been purified from cell culture supernatant using Protein G affinity chromatography.

Presentation
Vial containing 1 mg purified antibody in PBS pH 7.4. The concentration is given on the vial label. Spin the vial briefly before opening.

Storage and Stability
Store the antibody at 2°-8°C. For long-term storage the antibody should be aliquoted and stored at –20°C or colder. It is recommended to avoid freeze-thaw cycles.

Applications
ELISA
The antibody can be used as capture antibody in ELISAs. An antibody concentration of 1-10 µg/ml is recommended.
Immunocytochemistry
The antibody can be used for immune-cytochemistry on paraformaldehyde fixed spirochetes.
Westernblot
The antibody is suitable for Western blot analysis, detecting native and recombinant BpcA following SDS-PAGE under reducing conditions. A primary antibody concentration of 1-10 µg/mL is recommended.

Surface exposition of BpcA. Proteinase K and trypsin treatment affects surface expression of native BpcA. B. parkeri cells were incubated with the indicated concentrations of proteinase K and trypsin, lysed,  immunoblotted, and probed with anti-BpcA mAb Bp-3/32.2.

Category: Research use only

Type: Antibody

Product Availability: Worldwide

Manufacturer: ImmBioMed GmbH & Co KG, Germany

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