murine MAb against CbiA of Borrelia miyamotoi, Clone BM-C1-J12/6.7
Description
Tick-borne relapsing fever (TBRF) spirochetes include Borrelia (B.) hermsii, B. parkeri, B. duttonii, and B. miyamotoi. As an emerging human pathogen B. miyamotoi is transmitted, in contrast to other TBRF spirochetes, by hard-bodied ticks. B. miyamotoi occurs sympatrically with B. burgdorferi s.l. in Asia, North America, and Europe. Human cases were first reported in patients with nonspecific febrile illness and symptoms similar to relapsing fever, including high-grade fever, headache, fatigue, chills, myalgia, arthralgia, and nausea led to the implementation of a new entity termed hard tick-borne relapsing fever (HTBRF). B. miyamotoi efficiently evade complement via CbiA, exhibiting complement inactivating properties.
Properties
The monoclonal antibody ADG0174L (BM-C1-J12/6.7) is a murine monoclonal antibody, subclass IgG1 recognizing CbiA of Borrelia miyamotoi strain HT31. Mice were immunized with rec. CbiA. The antibody has been purified from cell culture supernatant using Protein G affinity chromatography.
Presentation
Vial containing 1 mg purified antibody in PBS pH 7.4. The concentration is given on the vial label. Spin the vial briefly before opening.
Storage and Stability
Store the antibody at 2°-8°C. For long-term storage the antibody should be aliquoted and stored at –20°C or colder. It is recommended to avoid freeze-thaw cycles.
Applications
ELISA
The antibody can be used as capture antibody in ELISAs. An antibody concentration of 1-10 µg/ml is recommended.
Westernblot
The antibody is suitable for Western blot analysis, detecting native and recombinant CbiA following SDS-PAGE under reducing conditions. A primary antibody concentration of 1-10 µg/mL is recommended.
Immunocytochemistry
The antibody can be used for immune-cytochemistry on paraformaldehyde fixed spirochetes.
Category: Research use only
Type: Antibody
Product Availability: Worldwide
Manufacturer: ImmBioMed GmbH & Co KG, Germany
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murine MAb against CbiA of Borrelia miyamotoi, Clone BM-C1-J12/6.7
Cat.No. ADG0174L (research box)
Article no.: 938332
Unit: 1,0 mg
Code: ADG0174L
Manufacturer: ImmBioMed GmbH & Co. KG - from the research box
References
- The relapsing fever spirochete Borrelia miyamotoi resists complement-mediated killing by human serum. Teegler et al. Ticks Tick Borne Dis. 2014; 5(6):898-901
- Immune evasion of Borrelia miyamotoi: CbiA, a novel outer surface protein exhibiting complement binding and inactivating properties. Rötterding et al. Sci. Rep. 2017; 7(1):303
- The complement binding and inhibitory protein CbiA of Borrelia miyamotoi degrades extracellular matrix components by interacting with plasmin(ogen). Nguyen et al. Front. Cell. Infect. Microbiol. 2018; 8:23
- Immune evasion strategies of relapsing fever spirochetes. Rötterding and Kraiczy. Front. Immunol. 2020; 11:1560