murine MAb against human SNF1LK, clone HD-pKe 122 8.3
Description
SNF1-like kinase belongs to the Ser/Thr protein kinase family. SNF1LK does not share functional homology with other SNF1-related kinases, but rather represents a new subclass with novel molecular activities. Recent data suggest a possible function for SNF1LK in G2/M regulation.
Preparation
The monoclonal antibody ADG0012 (clone HD-pKe 122 8.3) is a murine monoclonal antibody, subclass IgG1 recognizing the human SNF1-like kinase protein. Mice were immunized with recombinant SNF1-like kinase (expressed in E. coli). The antibody has been purified from cell culture supernatant using Protein G affinity chromato-graphy.
Presentation
Screw capped vial containing 250 µg of purified antibody in PBS pH 7.4 + 0.01% ProClin300. The IgG concentration is given on the vial label. Spin the vial briefly before opening.
Storage and Stability
Store the antibody at 2°-8°C. For long-term storage the antibody should be aliquoted and stored at –20°C or colder. It is recommended to avoid freeze-thaw cycles.
Applications
Western-Blot
![ADG0012_Westernblot](/uploads/artikeldetails/938363_ADG0012_P1.jpg)
lane 1: in vitro synthesized SNF1-like kinase, reticulocyte-lysate
lane 2: cultured normal keratinocytes 4 hours after detachment from the growth substratum, cell lysate
Immunocytology
COS cells transfected with SNF1-like kinase
Immunohistology
![ADG0012_Immunohistology](/uploads/artikeldetails/938363_ADG0012_P3.jpg)
A: normal skin, B: lesional skin- bullous pemphigoid; (e) epidermis, (d) dermis
Category: Research use only
Type: Antibody
Product Availability: Worldwide
Manufacturer: ImmBioMed GmbH & Co KG, Germany
For more information please click .pdf icon below.
murine MAb against human SNF1LK, clone HD-pKe 122 8.3
Cat.No. ADG0012 (research box)
Article no.: 938363
Unit: 250 µg
Code: ADG0012
Manufacturer: ImmBioMed GmbH & Co. KG - from the research box
References
- snf1lk encodes a protein kinase that may function in cell cycle regulation. Stephenson A, Huang GY, Nguyen NT, Reuter S, McBride JL, Ruiz JC. Genomics. 2004 Jun;83(6):1105-15.