murine MAb against human SNF1LK, clone HD-pKe 122 8.3

Description
SNF1-like kinase belongs to the Ser/Thr protein kinase family. SNF1LK does not share functional homology with other SNF1-related kinases, but rather represents a new subclass with novel molecular activities. Recent data suggest a possible function for SNF1LK in G2/M regulation.

Preparation
The monoclonal antibody ADG0012 (clone HD-pKe 122 8.3) is a murine monoclonal antibody, subclass IgG₁ recognizing the human SNF1-like kinase protein. Mice were immunized with recombinant SNF1-like kinase (expressed in E. coli). The antibody has been purified from cell culture supernatant using Protein G affinity chromato-graphy.

Presentation
Screw capped vial containing 250 µg of purified antibody in PBS pH 7.4 + 0.01% ProClin300. The IgG concentration is given on the vial label. Spin the vial briefly before opening.

Storage and Stability
Store the antibody at 2°-8°C. For long-term storage the antibody should be aliquoted and stored at –20°C or colder. It is recommended to avoid freeze-thaw cycles.

Applications
Western-Blot

lane 1: in vitro synthesized SNF1-like kinase, reticulocyte-lysate
lane 2: cultured normal keratinocytes 4 hours after detachment from the growth substratum, cell lysate

Immunocytology
 
COS cells transfected  with SNF1-like kinase

Immunohistology

A: normal skin, B: lesional skin- bullous pemphigoid; (e) epidermis, (d) dermis

Category: Research use only

Type: Antibody

Product Availability: Worldwide

Manufacturer: ImmBioMed GmbH & Co KG, Germany

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