TFPI, human, recombinant
Description
Full-length recombinant human tissue factor pathway inhibitor (TFPI) with C-terminal His-Tag expressed in HEK cells. The protein was purified from serum-free cell culture media using affinity chromatography.
Intended Use
This product is intended to serve as a positive control for Western blot studies.
Properties
Although this product is intended for identity studies, this particular lot inhibited the catalytic activity of the TF/FVIIa complex to activate factor X to factor Xa (using the ACTICHROME® TFPI chromogenic activity kit, product no. 848).
Purity
Estimated at greater than 95% per SDS gel electrophoresis and coomassie staining. ADG49B appears as a single band at approximately 45 kDa.
![938509_ADG49](/uploads/artikeldetails/938509_adg49.jpg)
Presentation
Clear glass screw capped vial containing 5 µg of recombinant human TFPI lyophilized from 50 µl of 50 mM Tris/HCl, 0.1 M NaCl, pH 7.4 with 0.2 M Mannitol added as an excipient. No preservatives have been added.
Reconstitution
Add 50 µl of filtered deionized water to dissolve the protein and generate a 100 µg/ml solution.
When diluting TFPI below 100 µg/ml, it is recommended to add a carrier protein to prevent protein loss from sticking to glass surfaces.
Storage
Store lyophilized protein at 2°-8°C. Aliquot and store reconstituted protein in plastic tubes at -20 °C or colder. Avoid freeze-thaw cycles.
Category: Research use only
Type: Protein
Product Availability: Worldwide
Manufacturer: ImmBioMed GmbH & Co KG, Germany
For more information please click .pdf icon below.
TFPI, human, recombinant
Cat.No. ADG49
Article no.: 938509
Unit: 5 µg
Code: ADG49
Manufacturer: ImmBioMed GmbH & Co. KG
References
- Syndecan-3 and TFPI colocalize on the surface of endothelial-, smooth muscle-, and cancer cells. Tinholt M. et al., PLoS One. 2015 Jan 24;10(1): :e0117404